Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Prdx1 Antibody recognizes endogenous levels of total Prdx1 protein. This antibody does not cross-react with Prdx2 protein.
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro108 of human Prdx1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Prdx1 belongs to a family of non-seleno peroxidases which function as H2O2 scavengers. All 6 prdx isoforms share a conserved N-terminal cysteine (Cys51) which is oxidized by H2O2 to Cysteine-Sulfenic acid (Cys51-SOH) and, in turn, reacts with Cys172-SH of the other prdx forming a disulfide dimer that protects it from degradation (1-3). Abnormally high levels of H2O2 cause Prdx1 to form an oligomeric chaperon that loses its peroxidase activity (4). Prdxs have been reported to bind to JNK and c-Abl and regulate their kinase acitivity (5,6). Prdx1 was shown to bind to PTEN and regulate its phosphatase activity in conditions of mild or no cellular stress, hence preventing Akt-driven transformation by protecting PTEN from oxidation-induced inactivation (7).
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