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Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Atg101 Antibody recognizes endogenous levels of total Atg101 protein. This antibody also detects a 55 kDa protein of unknown origin in some cell lines.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val177 of human Atg101 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Atg101 was discovered as a binding protein for Atg13, a component of the ULK1 serine-threonine kinase complex required for autophagy (1-3). Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (4,5). It is generally activated by conditions of nutrient deprivation, but is also associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (6). The molecular machinery of autophagy was largely discovered in yeast and is directed by a number of autophagy-related (Atg) genes. The ULK1 complex includes both Atg13 and FIP200 and is required for starvation-induced autophagy (7-9). Interaction between Atg101 and Atg13 can be important for the stability and basal phosphorylation of Atg13 and ULK1 (1,2).

1.  Mercer, C.A. et al. (2009) Autophagy 5, 649-62.

2.  Hosokawa, N. et al. (2009) Autophagy 5, 973-9.

3.  Chan, E.Y. et al. (2007) J Biol Chem 282, 25464-74.

4.  Reggiori, F. and Klionsky, D.J. (2002) Eukaryot. Cell 1, 11-21.

5.  Codogno, P. and Meijer, A.J. (2005) Cell Death Differ. 12 Suppl 2, 1509-18.

6.  Levine, B. and Yuan, J. (2005) J. Clin. Invest. 115, 2679-88.

7.  Hosokawa, N. et al. (2009) Mol Biol Cell 20, 1981-91.

8.  Jung CH et al. (2009) Mol Biol Cell 20, 1992–2003

9.  Ganley, I.G. et al. (2009) J Biol Chem 284, 12297-305.

Entrez-Gene Id 60673
Swiss-Prot Acc. Q9BSB4

For Research Use Only. Not For Use In Diagnostic Procedures.
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Atg101 Antibody