Upstream / Downstream

pathwayImage

Explore pathways related to this product.

Our U.S. Offices Are Closed

Our U.S. offices are closed in observance of Memorial Day. We will reopen on Tuesday, May 30th.

Thank you for your patience.

Questions?

Find answers on our FAQs page.

ANSWERS  

PhosphoSitePlus® Resource

  • Additional protein information
  • Analytical tools

LEARN MORE

We recommend the following alternatives

W     IF F   H M R Mk
REACTIVITY SENSITIVITY MW (kDa) Isotype
Rabbit 

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D6G10) XP® Rabbit mAb detects endogenous levels of phosporylated Smad2 and Smad3. Some reactivity is observed with non-phosphorylated Smad2 and 3 by western blot analysis. This antibody does not cross-react with other Smad-related proteins.


Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey, Xenopus, Bovine

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser465/467 of human Smad2. This region is highly conserved with Smad3 at Ser423/425.

Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5, and 8; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7 (1-5). Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses (6-8).


Following stimulation by TGF-β, Smad2 and Smad3 become phosphorylated at their carboxyl termini (serines 465 and 467 on Smad2; serines 423 and 425 on Smad3) by the receptor kinase TGF-β R1(9-11). Following phosphorylation, Smad2 and Smad3 form a heteromeric complex with the co-smad family member Smad4. These complexes are translocated to the nucleus where they bind DNA and regulate gene transcription.


1.  Heldin, C.H. et al. (1997) Nature 390, 465-71.

2.  Attisano, L. and Wrana, J.L. (1998) Curr Opin Cell Biol 10, 188-94.

3.  Derynck, R. et al. (1998) Cell 95, 737-40.

4.  Massagué, J. (1998) Annu Rev Biochem 67, 753-91.

5.  Whitman, M. (1998) Genes Dev 12, 2445-62.

6.  Wu, G. et al. (2000) Science 287, 92-7.

7.  Attisano, L. and Wrana, J.L. (2002) Science 296, 1646-7.

8.  Moustakas, A. et al. (2001) J Cell Sci 114, 4359-69.

9.  Abdollah, S. et al. (1997) J. Biol. Chem. 272, 27678-27685.

10.  Souchelnytskyi, S. et al. (1997) J. Biol. Chem. 272, 28107-28115.

11.  Liu, X. et al. (1997) Proc. Natl. Acad. Sci. USA 94, 10669-10674.


Entrez-Gene Id 4087 , 4088
Swiss-Prot Acc. Q15796 , P84022


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.

9510
Phospho-Smad2 (Ser465/467)/ Smad3 (Ser423/425) (D6G10) XP® Rabbit mAb