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Phospho-Smad2 (Ser465/467)/ Smad3 (Ser423/425) (D6G10) XP® Rabbit mAb #9510
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|8828||Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb||H M R Mk|
Gallery: Phospho-Smad2 (Ser465/467)/ Smad3 (Ser423/425) (D6G10) XP® Rabbit mAb #9510
Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D6G10) XP® Rabbit mAb detects endogenous levels of phosporylated Smad2 and Smad3. Some reactivity is observed with non-phosphorylated Smad2 and 3 by western blot analysis. This antibody does not cross-react with other Smad-related proteins.Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey, Xenopus, Bovine
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser465/467 of human Smad2. This region is highly conserved with Smad3 at Ser423/425.
Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5, and 8; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7 (1-5). Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses (6-8).
Following stimulation by TGF-β, Smad2 and Smad3 become phosphorylated at their carboxyl termini (serines 465 and 467 on Smad2; serines 423 and 425 on Smad3) by the receptor kinase TGF-β R1(9-11). Following phosphorylation, Smad2 and Smad3 form a heteromeric complex with the co-smad family member Smad4. These complexes are translocated to the nucleus where they bind DNA and regulate gene transcription.
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. XP is a registered trademark of Cell Signaling Technology, Inc.