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9519
Smad6 Antibody

Smad6 Antibody #9519

This product is discontinued

Storage:

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Smad6 Antibody detects endogenous levels of total Smad6 protein.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding cysteine 55 of human Smad6. Antibodies were purified by protein A and peptide affinity chromatography.

Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5, and 8; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7 (1-5). Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses (6-8).

The inhibitory Smads, Smad6 and Smad7, were originally identified in vascular endothelium and inhibit TGF-β and BMP signaling by interfering with the phosphorylation of other Smad family members (9,10). Their expression may be induced by TGF-β, and BMP in some cell types providing a negative feedback loop (11,12).

  1. Heldin, C.H. et al. (1997) Nature 390, 465-71.
  2. Attisano, L. and Wrana, J.L. (1998) Curr Opin Cell Biol 10, 188-94.
  3. Derynck, R. et al. (1998) Cell 95, 737-40.
  4. Massagué, J. (1998) Annu Rev Biochem 67, 753-91.
  5. Whitman, M. (1998) Genes Dev 12, 2445-62.
  6. Wu, G. et al. (2000) Science 287, 92-7.
  7. Attisano, L. and Wrana, J.L. (2002) Science 296, 1646-7.
  8. Moustakas, A. et al. (2001) J Cell Sci 114, 4359-69.
  9. Topper, J.N. et al. (1997) Proc. Natl. Acad. Sci. USA 94, 9314-9319.
  10. Imamura, T. et al. (1997) Nature 389, 622-626.
  11. Takase, M. et al. (1998) Biochem. Biophys. Res. Commun. 244, 26-29.
  12. Afrakhte, M. et al. (1998) Biochem. Biophys. Res. Commun. 249, 505-511.
Entrez-Gene Id
4091
Swiss-Prot Acc.
O43541
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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