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Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

NT5E/CD73 Antibody recognizes endogenous levels of total NT5E protein.

Species predicted to react based on 100% sequence homology: Monkey

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp522 of human NT5E protein. Antibodies are purified by protein A and peptide affinity chromatography.

Ecto-5'-nucleotidase (NT5E, also called CD73) is a 70 kDa glycosyl phosphatidylinositol-anchored, membrane-bound glycoprotein that catalyzes the hydrolysis of extracellular nucleoside monophosphates into bioactive nucleosides (1). NT5E catalyzes the terminal step of extracellular adenosine formation from adenosine monophosphate, which drives the regulation of extracellular adenosine levels and the downstream activation of the four G protein-coupled adenosine receptors (2). Binding of hypoxia-inducible factor (HIF-1) to the NT5E gene promoter leads to upregulation of NT5E during hypoxia (3). The biological roles of NT5E include lymphocyte adhesion (4,5), fibrosis (6), and the regulation of nociception (7,8).

1.  Le Hir, M. and Kaissling, B. (1993) Am J Physiol 264, F377-87.

2.  Schulte, G. and Fredholm, B.B. (2003) Cell Signal 15, 813-27.

3.  Synnestvedt, K. et al. (2002) J Clin Invest 110, 993-1002.

4.  Arvilommi, A.M. et al. (1997) Eur J Immunol 27, 248-54.

5.  Airas, L. and Jalkanen, S. (1996) Blood 88, 1755-64.

6.  Peng, Z. et al. (2008) Nucleosides Nucleotides Nucleic Acids 27, 821-4.

7.  Sowa, N.A. et al. (2010) J Neurosci 30, 2235-44.

8.  Zylka, M.J. (2011) Trends Mol Med 17, 188-96.

Entrez-Gene Id 4907
Swiss-Prot Acc. P21589

For Research Use Only. Not For Use In Diagnostic Procedures.
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NT5E/CD73 Antibody