The Rag Protein Antibody Sampler Kit provides an economical means to evaluate the regulation of mTORC1 signaling by such proteins as Raptor, RagA, RagB, RagC, and RagD. The kit contains enough primary and secondary antibodies to perform four Western blot experiments per primary antibody.
Each antibody in the Rag Protein Antibody Sampler Kit detects endogenous levels of its target protein. RagA (D8B5) Rabbit mAb may also detect endogenous levels of total RagB protein.
Polyclonal antibody is produced by immunizing animal with synthetic peptide corresponding to residues in the human sequence for RagD proteins. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues of human mTOR, RagA, and Raptor, and corresponding to residues near the amino terminus of human RagB, and RagC protein.
The mTORC1 kinase complex is a critical regulator of cell growth (1,2). Its activity is modulated by energy levels, growth factors, and amino acids via signaling through Akt, MAPK, and AMPK pathways (3,4). Recent studies found that the four related GTPases, RagA, RagB, RagC, and RagD, interact with raptor within the mTORC1 complex (1,2). These interactions are both necessary and sufficient for mTORC1 activation in response to amino acid signals (1,2).
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
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