The Gab Antibody Sampler Kit provides an economical means of evaluating levels of Gab1 and Gab2 total protein, as well phosphorylated Gab1 and Gab2 at the specified sites. The kit contains enough reagents to perform four western blot experiments per primary antibody.
Gab1 Antibody detects endogenous levels of total Gab1 protein. Gab2 Antibody detects endogenous levels of total Gab2 protein. Each activation-state antibody detects endogenous levels of Gab1 or Gab2 phosphorylated at the specified sites. There may be some cross-reactivity with other phosphorylated proteins. Please consult individual product web pages for more details.
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr472 of Gab1, or phosphopeptides corresponding to residues surrounding Tyr307 of human Gab1 or Ser159 of human Gab2. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.
Monoclonal antibodies are produced by immunizing animals with recombinant human Gab2, or synthetic phosphopeptides corresponding to residues surrounding Tyr627 of human Gab1 or Tyr452 of human Gab2.
The Grb-associated binder (Gab) family is a family of adaptor proteins recruited by a wide variety of receptor tyrosine kinases (RTKs) such as EGFR, HGFR, insulin receptor, cytokine receptor and B cell antigen receptors. Upon stimulation of RTKs by their cognate ligand, Gab is recruited to the plasma membrane where it is phosphorylated and functions as a scaffold (1-4). Multiple tyrosine phosphorylation sites of Gab1 protein have been identified (5). Phosphorylation of Tyr472 regulates its binding to p85 PI3 kinase (6,7). Phosphorylation of Gab1 at Tyr307, Tyr373 and Tyr407 modulates its association to PLCγ (8). Phosphorylation of Tyr627 and Tyr659 is required for Gab1 binding to and activation of the protein tyrosine phosphatase SHP2 (6,9).
Gab2, a recently identified docking protein of Gab family, contains a pleckstrin homology domain and potential binding sites for SH2 and SH3 domain-containing proteins. Gab2 has been shown to support growth, differentiation and function in a number of hematopoietic cells (10).
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
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