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PhosphoSitePlus® Resource

  • Additional protein information
  • Analytical tools


Product Description

The c-Cbl Antibody Sampler Kit provides an economical means of evaluating levels of c-Cbl protein phosphorylated at the specified sites, as well as total c-Cbl levels. The kit contains enough reagents to perform four western blot experiments per primary antibody.

Specificity / Sensitivity

The Phospho-c-Cbl (Tyr700) and (Tyr731) Antibodies detect endogenous c-Cbl when phosphorylated at the specified sites. c-Cbl (C49H3) Rabbit mAb detects endogenous levels of total c-Cbl protein.

Source / Purification

The phospho-specific polyclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr731 of human c-Cbl. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. The monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to the sequence of human c-Cbl and residues surrounding Tyr700 of human c-Cbl.

The c-Cbl proto-oncogene is a ubiquitously expressed cytoplasmic adaptor protein that is especially predominant in hematopoietic cells (1,2). c-Cbl is rapidly tyrosine-phosphorylated in response to stimulation of a variety of cell-surface receptors and becomes associated with a number of intracellular signaling molecules such as protein tyrosine kinases, phosphatidylinositol-3 kinase, Crk, and 14-3-3 proteins (3,4). c-Cbl possesses a highly conserved amino-terminal phosphotyrosine binding domain (TKB) and a C3HC4 RING finger motif. The TKB recognizes phosphorylated tyrosines on activated receptor tyrosine kinases (RTKs) as well as other nonreceptor tyrosine kinases. The RING finger motif recruits ubiquitin-conjugating enzymes. These two domains are primarily responsible for the ubiquitin ligase activity of c-Cbl and downregulation of RTKs (3). Research studies have indicated that in human cancer tissues, c-Cbl is frequently tyrosine-phosphorylated in a tumor-specific manner (5). Phosphorylation of Tyr731 of c-Cbl provides a docking site for downstream signaling components such as p85 and Fyn (6).

1.  Blake, T.J. et al. (1991) Oncogene 6, 653-657.

2.  Thien, C.B. and Langdon, W.Y. (1998) Immunol. Cell Biol. 76, 473-482.

3.  Christine, B.F. et al. (2001) Nat. Rev. Mol. Cell Biol. 2, 294-307.

4.  Feshchenko, E.A. et al. (1998) J. Biol. Chem. 273, 8323-8331.

5.  Kamei, T. et al. (2000) Int. J. Oncol. 17, 335-339.

6.  Hunter, C. et al. (1999) J. Biol. Chem. 274, 2097-2106.

Entrez-Gene Id 867
Swiss-Prot Acc. P22681

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.

c-Cbl Antibody Sampler Kit