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Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Glutamate Dehydrogenase 1/2 Antibody recognizes endogenous levels of total glutamate dehydrogenase 1 and 2 proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn339 of human glutamate dehydrogenase 1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Glutamate dehydrogenase is a mitochondrial enzyme that catalyzes the oxidative deamination of glutamate to α-ketoglutarate through association with the cofactor nicotinamide adenine dinucleotide phosphate (1). Glutamate dehydrogenase is highly expressed in various tissues such as the liver, brain, kidney, heart, pancreas, ovaries, and testis. Two isoforms produced by two distinct genes are found in mammalian tissues. The GLUD1 gene is ubiquitously expressed (2), while the GLUD2 gene is specifically expressed in testicular tissues and astrocytes (3,4). Glutamate dehydrogenase links glutamate to the Krebs cycle, thereby playing a critical role in the regulation of energy homeostasis. Research studies have shown that changes in glutamate dehydrogenase activity in pancreatic β-cells can cause a hyperinsulinism syndrome (5).

1.  Blumenthal, K.M. et al. (1975) J Biol Chem 250, 3644-54.

2.  Michaelidis, T.M. et al. (1993) Genomics 16, 150-60.

3.  Shashidharan, P. et al. (1997) J Neurochem 68, 1804-11.

4.  Zaganas, I. et al. (2012) Neurochem Int 61, 455-62.

5.  Karaca, M. et al. (2011) Neurochem Int 59, 510-7.

Entrez-Gene Id 2746
Swiss-Prot Acc. P00367

For Research Use Only. Not For Use In Diagnostic Procedures.
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Glutamate Dehydrogenase 1/2 Antibody