Revision 1

#10893

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IP

Reactivity:
H M R

Sensitivity:
Endogenous

MW (kDa):
82

Source/Isotype:
Rabbit IgG

UniProt ID:
#Q7Z417

Entrez-Gene Id:
57532

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity/Sensitivity

NUFIP2 (F5O7H) Rabbit Monoclonal Antibody recognizes endogenous levels of total NUFIP2 protein. This antibody also reacts with a protein at 65 kDa, which likely represents post-translational modification of NUFIP2.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human NUFIP2 protein.

Background

Nuclear FMR1-interacting protein 2 (NUFIP2) was initially identified and characterized as a novel RNA-binding protein associated with the fragile X mental retardation protein (FMRP) (1). NUFIP2 functions as a ribonucleoprotein large subunit assembly factor and facilitates ribosome biogenesis through binding of specific components necessary for protein synthesis (1). As one of many FMRP-interacting proteins, NUFIP2 localizes to several subcellular compartments, including the cytoplasmic stress granule, nuclear body, and ribosome, indicating involvement in both nuclear and cytoplasmic processes (2). Moreover, the subcellular distribution of NUFIP2 is cell cycle-dependent in cultured cells, suggesting that the composition of FMRP-containing RNP complexes may be cell cycle-modulated (1). Under lysosomal-damaging conditions, NUFIP2 directly interacts with GABARAPs of the ATG8 family. Subsequent Atg8ylation of NUFIP2 drives its recruitment to stress granules where it contributes to mTOR inactivation via the Ragulator–RagA/B complex (3,4). NUFIP2 has also been identified as a regulatory cofactor of Roquin-1 and Roquin-2, RNA-binding proteins which recognize secondary structures in target mRNA 3′-UTRs and induce mRNA decay (5).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting IP: Immunoprecipitation

Cross-Reactivity Key

H: Human M: Mouse R: Rat

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of extracts from various cell lines using NUFIP2 (F5O7H) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). U-2 OS cells are negative for NUFIP2 expression due to a deletion within the NUFIP2 gene.
Western Blotting Image 1: NUFIP2 (F5O7H) Rabbit Monoclonal Antibody
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human NUFIP2 protein (hNUFIP2-Myc/DDK; +), using NUFIP2 (F5O7H) Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Western Blotting Image 2: NUFIP2 (F5O7H) Rabbit Monoclonal Antibody
Immunoprecipitation of NUFIP2 protein from KYSE-180 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is NUFIP2 (F5O7H) Rabbit mAb. Western blot analysis was performed using NUFIP2 (F5O7H) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody.
Immunoprecipitation Image 1: NUFIP2 (F5O7H) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.