Product Pathways - Neuroscience
PC1/3 Antibody #11914
|11914S||100 µl (10 western blots)||---||In Stock||---|
|11914||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat||Endogenous||70||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
PC1/3 Antibody recognizes endogenous levels of total PC1/3 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg364 of human PC1/3 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from mouse cortex, wild-type (WT) and PC1/3 (-/-) mouse hypothalamus, and human hypothalamus using PC1/3 Antibody (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Tissues from WT and PC1/3 (-/-) mice were kindly provided by Dr. Robert Day (University of Sherbrooke, Quebec, Canada).
The proprotein convertases (PCs) are enzymes that activate precursor proteins through proteolytic cleavage within the secretory pathway. PCs comprise several enzymes that are basic amino acid-specific proteinases (furin, PC1/3, PC2, PC4, PACE4, PC5/6, and PC7), as well as nonbasic amino acid convertases (S1P and PC9) (1). PCs have a common structure that includes an N-terminal signal peptide for secretory pathway targeting; a pro-domain that is thought to act as an intramolecular chaperone; a catalytic domain containing the active site; a P-domain that contributes to the overall folding of the enzyme by regulating stability, calcium-, and pH-dependence; and a C-terminal domain that interacts with the membrane (2). PCs act in a tissue- and substrate-specific fashion to generate an array of bioactive peptides and proteins from precursors, both in the brain and the periphery (3).
Research studies examining the PCSK1 gene (encoding PC1/3) suggest that mutations that cause impaired PC1/3 expression or enzymatic activity contribute to obesity (4).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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