Product Pathways - Ca / cAMP / Lipid Signaling
Phospho-MARCKS (Ser159/163) (D13D2) Rabbit mAb #11992
|11992S||100 µl (10 western blots)||---||In Stock||---|
|11992||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat||Endogenous||75 (rodent), 80 (human)||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Species predicted to react based on 100% sequence homology: Monkey, Chicken, Xenopus, Zebrafish, Bovine, Pig.
Specificity / Sensitivity
Phospho-MARCKS (Ser159/163) (D13D2) Rabbit mAb recognizes endogenous levels of MARCKS protein when phosphorylated at Ser159 and Ser163 (human residues).
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser159 and Ser163 of human MARCKS protein.
Western blot analysis of extracts from HeLa, NIH/3T3, and C6 cells, serum starved overnight and either DMSO-treated or treated with TPA #4174 (200 nM, 15 min), using Phospho-MARCKS (Ser159/163) (D13D2) Rabbit mAb.
Immunoprecipitation of phospho-MARCKS (Ser159/163) from HeLa cells, either DMSO-treated or treated with TPA #4174 (200 nM, 15 min), using Phospho-MARCKS (Ser159/163) (D13D2) Rabbit mAb (lanes 3 and 4) or MARCKS (D88D11) XP® Rabbit mAb #5607 (lanes 5 and 6). Lanes 1 and 2 represent 10% of input lysate for each treatment. Western blot analysis was performed using MARCKS (D88D11) XP® Rabbit mAb #5607. Note: MARCKS (D88D11) XP® Rabbit mAb #5607 does not immunoprecipitate phospho-MARCKS (Ser159/163) and was used as a negative control.
Myristoylated Alanine-Rich C-Kinase Substrate (MARCKS) is a major PKC substrate expressed in many cell types. MARCKS has been implicated in cell motility, cell adhesion, phagocytosis, membrane traffic, and mitogenesis (1). PKC phosphorylates Ser159, 163, 167, and 170 of MARCKS in response to growth factors and oxidative stress. Phosphorylation at these sites regulates the calcium/calmodulin binding and filamentous (F)-actin cross-linking activities of MARCKS (2-4). Phosphorylation by PKC also results in translocation of MARCKS from the plasma membrane to the cytoplasm (5).
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