Product Pathways - Protein Stability
PSMA3 (D4Y9O) Rabbit mAb #12446
|12446S||100 µl (10 western blots)||---||In Stock||---|
|12446||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||28||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Species predicted to react based on 100% sequence homology: Dog, Pig, Horse.
Specificity / Sensitivity
PSMA3 (D4Y9O) Rabbit mAb recognizes endogenous levels of total PSMA3 protein. This antibody does not cross-react with other α subunits of the 20S proteasome. This antibody cross-reacts with a 140 kDa protein of unknown identity in lysates from certain cell lines.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg100 of human PSMA3 protein.
Western blot analysis of extracts from various cell lines using PSMA3 (D4Y9O) Rabbit mAb.
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human PSMA1 (hPSMA1-Myc/DDK; +), full-length human PSMA2 (hPSMA2-Myc/DDK; +), full-length human PSMA3 (hPSMA3-Myc/DDK; +), full-length human PSMA4 (hPSMA4-Myc/DDK; +), full-length human PSMA6 (hPSMA6-Myc/DDK; +), or full-length human PSMA7 (hPSMA7-Myc/DDK; +), using PSMA3 (D4Y9O) Rabbit mAb (upper) or DYKDDDDK Tag Antibody #2368 (lower).
Immunoprecipitation of PSMA3 from 293T cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or PSMA3 (D4Y9O) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using PSMA3 (D4Y9O) Rabbit mAb.
The 26S proteasome is a highly abundant, ~2 MDa complex that serves as the proteolytic arm of the ubiquitin-proteasome system. It consists largely of two sub-complexes, the 19S regulatory particle (RP) and the 20S catalytic core particle (CP); in many cases two RPs cap either end of a CP. The CP is made of two stacked β-rings that contain the catalytic sites, each of which is made of seven subunits (β1-7), flanked on either side by two α-rings, which are also made of seven subunits each (α1-7). Thus, the structure of the 20S CP is α1-7β1-7β1-7α1-7. The RP includes a base and a lid. The base, in part, is composed of a hexametric ring of ATPases that function to unfold the substrate and open the gate of the interlacing amino-terminal segments of the α-subunits, thus allowing entry of the unfolded substrate into the catalytic chamber. The lid is predominantly involved in specific recognition of the ubiquitin signal (1). In addition to the 19S cap, other proteins and complexes, such as proteasome activator 28 (PA28/11S), bind to the end of the 20S cylinder and activate it by facilitating opening of the gate. Furthermore, proteasome-associated DUBs and E3s can remodel substrate-anchored polyubiquitin chains, which may modulate their susceptibility to degradation (2).
PSMA3 (HC8/α7) is an α subunit of the 20S proteasome. PSMA3 lacks any known proteolytic activity but may have an important role in the intiation of ring formation and 20S proteasome assembly (3). Research studies have demonstrated that PSMA3 serves as a receptor within the proteasome, recruiting substrates that are destined for degradation in a ubiquitination-independent manner (4,5).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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