Product Pathways - Metabolism
SHMT1 Antibody #12612
|12612S||100 µl||---||In Stock||---|
|12612||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||50||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
SHMT1 Antibody recognizes endogenous levels of total SHMT1 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human SHMT1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing either DDK-tagged full-length human SHMT1 (hSHMT1-DDK; +) or DDK-tagged full-length human SHMT2 (hSHMT2-DDK; +), using SHMT1 Antibody (upper), DYKDDDDK Tag (9A3) Mouse mAb #8146 (middle), or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Immunoprecipitation of SHMT1 from extracts of HeLa cells using Normal Rabbit IgG #2729 (lane 2) or SHMT1 Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using SHMT1 Antibody. Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb #3677 and Anti-mouse IgG, HRP-linked Antibody #7076 were used as secondary antibodies.
Serine hydroxymethyltransferases 1 and 2 (SHMT1, SHMT2) are cytoplasmic and mitochondrial serine hydroxylmethyltransferases, respectively (1,2). They catalyze the conversion of serine to glycine with the transfer of β-carbon from serine to tetrahydrofolate (THF) to form 5, 10-methylene-THF (1, 2). Research studies indicate that SHMT1 hemizygosity is associated with higher risk of intestinal cancer in mice of a certain genetic background (3). Suppression of SHMT2 was shown to block cell proliferation (4).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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