Product Pathways - Lymphocyte Signaling
FoxP3 (D6O8C) Rabbit mAb #12632
|12632S||100 µl (10 western blots)||---||In Stock||---|
|12632||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, F=Flow Cytometry
* Product-specific protocol.
Specificity / Sensitivity
FoxP3 (D6O8C) Rabbit mAb recognizes endogenous levels of total FoxP3 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro293 of human FoxP3 protein.
Western blot analysis of extracts from COS-7 cells, mock transfected (-) or transfected with human FoxP3 (hFoxP3; +), and human thymus using FoxP3 (D8O6C) Rabbit mAb.
Flow cytometric analysis of human peripheral blood mononuclear cells gated on CD4+ lymphocytes, showing FoxP3 expression in CD25+ cells using FoxP3 (D6O8C) Rabbit mAb (left), and corresponding absence of signal in CD25+ cells using concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, (right). Anti-rabbit IgG (H+L), (F(ab')2) Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. Refer to the product specific protocol link indicated on the datasheet.
Forkhead box (Fox) proteins are a family of evolutionarily conserved transcription factors containing a sequence known as Forkhead box or winged helix DNA binding domain (1). The human genome contains 43 Fox proteins that are divided into subfamilies. The FoxP subfamily has four members, FoxP1 - FoxP4, which are broadly expressed and play important roles in organ development, immune response and cancer pathogenesis (2-4). The FoxP subfamily has several characteristics that are atypical among Fox proteins: their Forkhead domain is located at the carboxy-terminal region and they contain motifs that promote homo- and heterodimerization. FoxP proteins usually function as transcriptional repressors (4,5).
FoxP3 is crucial for the development of T cells with regulatory properties (Treg) (6). Mutations in the corresponding FoxP3 gene are associated with immunodeficiency polyendocrinopathy, enteropathy, and X-linked syndrome (IPEX) (7), while overexpression in mice causes severe immunodeficiency (8). Research studies have shown that FoxP3 functions as a tumor suppressor in several types of cancer (9-11).
- Myatt, S.S. and Lam, E.W. (2007) Nat Rev Cancer 7, 847-59.
- Shu, W. et al. (2001) J Biol Chem 276, 27488-97.
- Lu, M.M. et al. (2002) Gene Expr Patterns 2, 223-8.
- Koon, H.B. et al. (2007) Expert Opin Ther Targets 11, 955-65.
- Li, S. et al. (2004) Mol Cell Biol 24, 809-22.
- Ochs, H.D. et al. (2007) Immunol Res 38, 112-21.
- Bennett, C.L. et al. (2001) Nat Genet 27, 20-1.
- Kasprowicz, D.J. et al. (2003) J Immunol 171, 1216-23.
- Zuo, T. et al. (2007) Cell 129, 1275-86.
- Zuo, T. et al. (2007) J Clin Invest 117, 3765-73.
- Wang, L. et al. (2009) Cancer Cell 16, 336-46.
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