Product Pathways - Metabolism
SignalSilence® PFKP siRNA I #12649
|12649S||300 µl (3 nmol)||---||In Stock||---|
|12649||carrier free and custom formulation / quantity||email request|
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Species predicted to react based on 100% sequence homology: Monkey.
Western blot analysis of extracts from 293T cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® PFKP siRNA I (+), using PFKP (D2E5) Rabbit mAb #12746 (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The PFKP (D2E5) Rabbit mAb confirms silencing of PFKP expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control.
SignalSilence® PFKP siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit PFKP expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions For Use
CST recommends transfection with 100 nM SignalSilence® PFKP siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.
Each vial contains the equivalent of 100 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 μl per well.
Phosphofructokinase (PFK) catalyzes the phosphorylation of fructose-6-phosphate in glycolysis (1). There are three isozymes: muscle-type, liver-type, and platelet-type (2,3). Platelet-type phosphofructokinase (PFKP) is expressed in various cell types (4,5). Research studies have shown that genetic variations in PFKP are associated with individuals born small for gestational age that are prone to obesity and diabetes later in adulthood (6).
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- Morrison, N. et al. (1992) Hum Genet 89, 105-6.
- Vora, S. (1983) Isozymes Curr Top Biol Med Res 11, 3-23.
- Morgan, A.R. et al. (2010) BMC Med Genet 11, 125.
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For Research Use Only. Not For Use In Diagnostic Procedures.
SignalSilence® is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.