Product Pathways - Tyrosine Kinase / Adaptors
PDGF Receptor Activation Antibody Sampler Kit #12651
|12651S||1 Kit (8 x 40 µl)||---||In Stock||---|
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|Kit Includes||Quantity||Applications||Reactivity||Homology†||MW (kDa)||Isotype|
|Phospho-PDGF Receptor β (Tyr751) (C63G6) Rabbit mAb #4549||40 µl||W||H, M||R||190||Rabbit IgG|
|PDGF Receptor β (28E1) Rabbit mAb #3169||40 µl||W, IP, IHC-P, IHC-F, IF-IC||H, M, R||190||Rabbit IgG|
|Phospho-SHP-2 (Tyr542) Antibody #3751||40 µl||W, IP||H, M, R||Mk, C, X||72||Rabbit|
|SHP-2 Antibody #3752||40 µl||W||H, M, R||72||Rabbit|
|Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060||40 µl||W, IP, IHC-P, IHC-F, IF-IC, F||H, M, R, Hm, Mk, Dm, Z, B||C, X, Dg, Pg||60||Rabbit IgG|
|Akt (pan) (C67E7) Rabbit mAb #4691||40 µl||W, IP, IHC-P, IF-IC, F||H, M, R, Mk, Dm||Pg||60||Rabbit IgG|
|Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370||40 µl||W, IP, IHC-P, IF-IC, F||H, M, R, Hm, Mk, Mi, Dm, Z, B, Dg, Pg, Sc||C, Ce||44, 42||Rabbit IgG|
|p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695||40 µl||W, IP, IHC-P, IF-IC, F||H, M, R, Hm, Mk, Mi, Dm, Z, B, Dg, Pg, Ce||C||42, 44||Rabbit|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||Goat|
†Species predicted to react based on 100% sequence homology.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IHC-F=Immunohistochemistry (Frozen), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Reactivity Key: H=Human, M=Mouse, R=Rat, Hm=Hamster, Mk=Monkey, Dm=D. melanogaster, Z=Zebrafish, B=Bovine, Mi=Mink, Dg=Dog, Pg=Pig, Sc=S. cerevisiae, Ce=C. elegans
Western blot analysis of extracts from Rat2, C6, and NIH/3T3 cells using PDGF Receptor β (28E1) Rabbit mAb #3169.
Western blot analysis of extracts from C6 and NIH/3T3 cells, starved for 18 hours and either untreated (-) or PDGF-treated (50 ng/ml, 20 min; +), using Phospho-SHP-2 (Tyr542) Antibody #3751 (upper) or control SHP-2 Antibody #3752 (lower).
Western blot analysis of extracts from various cell lines using SHP-2 Antibody #3752.
Western blot analysis of extracts from PC-3 cells, untreated or LY294002/wortmannin-treated, and NIH/3T3 cells, serum-starved or PDGF-treated, using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 (upper) or Akt (pan) (C67E7) Rabbit mAb #4691 (lower).
Western blot analysis of extracts from 293, NIH/3T3 and C6 cells, treated with λ phosphatase or TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174 as indicated (+), using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370 (upper) and p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 (lower).
Western blot analysis of extracts from NIH/3T3 cells, untreated (-) or stimulated with Platelet-Derived Growth Factor (PDGF) #9909 (+), using Phospho-PDGF Receptor-β (Tyr751) (C63G6) Rabbit mAb #4549 (upper) or PDGF Receptor-β (2B3) Mouse mAb #3175 (lower).
Western blot analysis of recombinant Akt1, Akt2 and Akt3 proteins, and extracts from various cell lines, using Akt (pan) (C67E7) Rabbit mAb #4691.
The PDGF Receptor Activation Antibody Sampler Kit provides an economical means to evaluate the activation status of multiple members of the PDGF receptor pathway, including SHP-2, Akt, and p44/42 MAPK (Erk1/2). The kit includes enough antibody to perform four western blot experiments per primary antibody.
Specificity / Sensitivity
Unless otherwise indicated, each antibody in the PDGF Receptor Activation Antibody Sampler Kit recognizes endogenous levels of its specific target. Activation state antibodies detect their intended targets only when phosphorylated at the indicated site. Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb detects endogenous levels of p44 and p42 MAP kinase when dually phosphorylated at Thr202 and Tyr204 of Erk1 (Thr185/Tyr187 of Erk2) and singly phosphorylated at Thr202. Phospho-PDGF Receptor β (Tyr751) (C63G6) Rabbit mAb may cross-react with activated PDGF receptor α and other protein tyrosine kinases when highly overexpressed. PDGF Receptor β (28E1) Rabbit mAb may cross-react with PDGF receptor α when highly overexpressed. Phospho-SHP-2 (Tyr542) Antibody may cross-react with activated receptor tyrosine kinases.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Tyr542 of human SHP-2 protein or synthetic peptides corresponding to residues surrounding the amino-terminal domain of human SHP-2. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. Monoclonal activation state antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser473 of human Akt, Thr202/Tyr204 of human p44 MAP kinase, or Tyr751 of human PDGF receptor β. Monoclonal control antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues in the carboxy-terminal sequence of mouse Akt, the carboxy terminus of rat p44 MAP kinase, or a fusion containing a carboxy-terminal fragment of human PDGF receptor β protein.
Platelet derived growth factor (PDGF) family proteins form dimers (PDGF AA, PDGF AB, PDGF BB, PDGF CC, and PDGF DD) that bind receptor tyrosine kinases PDGF receptor α (PDGFRα) and PDGF receptor β (PDGFRβ) in a specific pattern. PDGFRβ homodimers bind PDGF BB and DD homodimers and the PDGF AB heterodimer. Heteromeric receptor PDGF α/β binds PDGF B, C, and D homodimers and the PDGF AB heterodimer (1). Ligand binding induces PDGF receptor dimerization and autophosphorylation, followed by binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules, such as GRB2, Src, GAP, PI3 kinase, PLCγ, and NCK. Activated PDGF receptors initiate signaling pathways that control cell growth, actin reorganization, migration, and differentiation (2). PDGFRβ kinase-insert region residue Tyr751 forms the PI3 kinase docking site, and phosphorylation of PDGFRβ at this site inhibits the association between the SH2 domain of the PI3 kinase p85 subunit and PDGFRβ (3,4).
SHP-2 (PTPN11) is a nonreceptor protein tyrosine phosphatase that participates in signaling pathways that control cell growth, differentiation, migration, and death (5). Activation of SHP-2 and its association with Gab1 is critical for sustained Erk activation downstream of growth factor receptors and cytokines (6). Phosphorylation of SHP-2 at Tyr542 and Tyr580 in response to growth factor receptor activation is thought to relieve basal inhibition and stimulate SHP-2 tyrosine phosphatase activity (7,8).
Insulin and various growth/survival factors activate Akt, a kinase that acts in a wortmannin-sensitive pathway involving PI3 kinase to help control survival and apoptosis (9-11). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (12) and by phosphorylation within the carboxy terminus at Ser473.
The p44/42 MAPK (Erk1/2) signaling pathway is activated in response to extracellular stimuli including mitogens, growth factors, and cytokines (13-15). Research suggests that this pathway is an important target in cancer diagnosis and treatment (16). External stimuli lead to activation of a kinase cascade that results in the activation of p44 and p42 by a MAP kinase. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively.
Clinical studies describe PDGF expression in a number of different solid tumors, from glioblastomas to prostate carcinomas. The biological role of PDGF signaling in these tumors varies from autocrine stimulation of cancer cell growth to more subtle paracrine interactions involving adjacent stroma and even angiogenesis. Targeting PDGF signaling may be an effective way for tumor treatment (17).
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- Maroun, C.R. et al. (2000) Mol Cell Biol 20, 8513-25.
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- Lu, W. et al. (2001) Mol Cell 8, 759-69.
- Franke, T.F. et al. (1997) Cell 88, 435-7.
- Burgering, B.M. and Coffer, P.J. (1995) Nature 376, 599-602.
- Franke, T.F. et al. (1995) Cell 81, 727-36.
- Alessi, D.R. et al. (1996) EMBO J 15, 6541-51.
- Roux, P.P. and Blenis, J. (2004) Microbiol Mol Biol Rev 68, 320-44.
- Baccarini, M. (2005) FEBS Lett 579, 3271-7.
- Meloche, S. and Pouysségur, J. (2007) Oncogene 26, 3227-39.
- Roberts, P.J. and Der, C.J. (2007) Oncogene 26, 3291-310.
- George, D. (2001) Semin Oncol 28, 27-33.
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* Product-specific protocol.
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For Research Use Only. Not For Use In Diagnostic Procedures.
XP® is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
Select rabbit monoclonal antibodies are developed, validated, and produced at CST using in part technology under license (granting certain rights including those under U.S. Patents No. 5,675,063 and in some instances 7,429,487) from Epitomics, Inc.