Product Pathways - NF-kB Signaling
Toll-like Receptor 6 (D1Z8B) Rabbit mAb #12717
|12717S||100 µl (10 western blots)||---||In Stock||---|
|12717||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse||Endogenous||90-110||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
TLR6 (D1Z8B) Rabbit mAb recognizes endogenous levels of total TLR6 protein. This antibody cross-reacts with a 72 kDa protein of unknown origin. This antibody is not approved for IP in mouse samples.
Source / Purification
Monoclonal antibody is produced by immunizing animals with synthetic peptides corresponding to residues surrounding Pro47 of human and mouse TLR6 protein.
Western blot analysis of extracts from THP-1 cells differentiated overnight with 80 nM TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174 and Raw 264.7 cells using Toll-like Receptor 6 (D1Z8B) Rabbit mAb.
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing HA-tagged full-length human TLR6 (hTLR6-HA; +) or transfected with a construct expressing HA-tagged full-length mouse TLR6 (mTLR6-HA; +), using Toll-like Receptor 6 (D1Z8B) Rabbit mAb.
Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in Drosophila, play a pivotal role in innate immune responses (1-4). TLRs recognize conserved motifs found in various pathogens and mediate defense responses (5-7). Triggering of the TLR pathway leads to the activation of NF-κB and subsequent regulation of immune and inflammatory genes (4). The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the Toll/Interleukin-1 receptor (TIR) domain (1). Upon activation, TLRs associate with a number of cytoplasmic adaptor proteins containing TIR domains, including myeloid differentiation factor 88 (MyD88), MyD88-adaptor-like/TIR-associated protein (MAL/TIRAP), Toll-receptor-associated activator of interferon (TRIF), and Toll-receptor-associated molecule (TRAM) (8-10). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK (8,11-14). Activation of IKK leads to the degradation of IκB, which normally maintains NF-κB in an inactive state by sequestering it in the cytoplasm.
Toll-like receptor 6 (TLR6) heterodimerizes with TLR2 and is expressed on the cell surface where it recognizes fungal zymosan and bacterial lipoproteins (15). In addition, a heterodimer of TLR4 and TLR6 was recently shown to assemble downstream of CD36 signaling and contribute to sterile inflammation in response to CD36 ligands, including low-density lipoprotein and β-amyloid (16).
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