Product Pathways - Protein Translation
NXF1 (D5X4G) Rabbit mAb #12735
|12735S||100 µl (10 western blots)||---||In Stock||---|
|12735||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||70||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Species predicted to react based on 100% sequence homology: Hamster, Bovine, Dog, Pig, Guinea Pig.
Specificity / Sensitivity
NXF1 (D5X4G) Rabbit mAb recognizes endogenous levels of total NXF1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln211 of human NXF1 protein.
mRNA export is a process that is tightly coupled to mRNA splicing (1-4). Splicing and packaging of mRNAs in the form of an mRNA-protein complex (mRNP) leads to the recruitment of the mRNA export adaptor THOC4/ALY, via its interaction with the splicing factor UAP56, forming a large complex termed the transcription-export complex (TREX) (1,2,5). THOC4/ALY then directly interacts with NXF1/TAP, a part of the heterodimer that targets the mRNP to the nuclear pore complex, resulting in the shuttling of mRNP out of the nucleus and into the cytoplasm (1-3,6).
- Katahira, J. (2012) Biochim Biophys Acta 1819, 507-13.
- Siddiqui, N. and Borden, K.L. (2012) Wiley Interdiscip Rev RNA 3, 13-25.
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- Masuda, S. et al. (2005) Genes Dev 19, 1512-7.
- Luo, M.L. et al. (2001) Nature 413, 644-7.
- Kang, Y. and Cullen, B.R. (1999) Genes Dev 13, 1126-39.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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