Product Pathways - Chromatin Regulation / Epigenetics
Chromodomain Helicase DNA-Binding (CHD) Family Antibody Sampler Kit #12775
|12775S||1 Kit (6 x 40 µl)||---||In Stock||---|
Already purchased this product? Write a Review.
|Kit Includes||Quantity||Applications||Reactivity||Homology†||MW (kDa)||Isotype|
|CHD1 (D8C2) Rabbit mAb #4351||40 µl||W, IP, ChIP||H, M, R, Mk||B, Dg, Pg||220||Rabbit IgG|
|CHD2 Antibody #4170||40 µl||W||H, M, R, Mk||260||Rabbit|
|CHD3 Antibody #4241||40 µl||W, IP||H, M, Mk||260||Rabbit|
|CHD4 Antibody #4245||40 µl||W||H, M, R||Mk, B, Hr||260||Rabbit|
|CHD7 (D3F5) Rabbit mAb #6505||40 µl||W, IP||H, M||336||Rabbit IgG|
|CHD8 (D3C1) Rabbit mAb #11891||40 µl||W, IP||H||290||Rabbit IgG|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||Goat|
†Species predicted to react based on 100% sequence homology.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, ChIP=Chromatin IP
Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey
Western blot analysis of extracts from OVCAR8 and SR cells using CHD8 (D3C1) Rabbit mAb #11891.
Western blot analysis of extracts from various cell lines using CHD2 Antibody #4170.
Western blot analysis of extracts from HeLa and Jurkat cells using CHD3 Antibody #4241.
Western blot analysis of extracts from 293 and NCCIT cells using CHD4 Antibody #4245.
Western blot analysis of extracts from various cell lines using CHD1 (D8C2) Rabbit mAb #4351.
Western blot analysis of extracts from DLD-1, SH-SY5Y and mES cells using CHD7 (D3F5) Rabbit mAb #6505.
The Chromodomain Helicase DNA-Binding (CHD) Family Antibody Sampler Kit provides an economical means to investigate the CHD family members associated with the histone deacetylase NuRD complex. This kit contains enough primary antibody to perform four western blots per primary antibody.
Specificity / Sensitivity
Each antibody in the Chromodomain Helicase DNA-Binding (CHD) Family Antibody Sampler Kit recognizes total endogenous levels of the specific target protein. CHD8 (D3C1) Rabbit mAb also cross-reacts with a protein of unknown origin at 140 kDa.
Source / Purification
Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues near the carboxy terminus of human CHD1 protein, the amino terminus of human CHD7 protein or the carboxy terminus of human CHD8 protein. Polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to human CHD3 protein, human CHD4 protein or residues surrounding Phe1579 of human CHD2 protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.
Chromodomain-helicase-DNA-binding domain (CHD) proteins have been identified in a variety of organisms (1,2). This family of nine proteins is divided into three separate subfamilies: subfamily I (CHD1 and CHD2), subfamily II (CHD3 and CHD4), and subfamily III (CHD5, CHD6, CHD7, CHD8, and CHD9). All CHD proteins contain two tandem amino-terminal chromodomains, a SWI/SNF-related ATPase domain, and a carboxy-terminal DNA-binding domain (1,2). The chromodomains facilitate binding to methylated lysine residues of histone proteins and confer interactions with specific regions of chromatin. The SWI/SNF-related ATPase domain utilizes energy from ATP hydrolysis to modify chromatin structure. CHD proteins are often found in large, multiprotein complexes with their transcriptional activation or repression activity governed by other proteins within the complex. CHD3 (also known as Mi2-α) and CHD4 (also known as Mi2-β) are central components of the nucleosome remodeling and histone deacetylase (NuRD) transcriptional repressor complex, which also contains HDAC1, HDAC2, RBAP48, RBAP46, MTA1, MTA2, MTA3, and MBD3 (3-8). Both CHD3 and CHD4 contain two plant homeodomain (PHD) zinc finger domains that bind directly to HDAC1 and HDAC2.
- Hall, J.A. and Georgel, P.T. (2007) Biochem Cell Biol 85, 463-76.
- Marfella, C.G. and Imbalzano, A.N. (2007) Mutat Res 618, 30-40.
- Tong, J.K. et al. (1998) Nature 395, 917-21.
- Xue, Y. et al. (1998) Mol Cell 2, 851-61.
- Zhang, Y. et al. (1998) Cell 95, 279-89.
- Bowen, N.J. et al. (2004) Biochim Biophys Acta 1677, 52-7.
- Jones, P.L. et al. (1998) Nat Genet 19, 187-91.
- Fujita, N. et al. (2003) Cell 113, 207-19.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.