Product Pathways - Metabolism
Glycolysis II Antibody Sampler Kit #12866
|12866S||1 Kit (8 x 40 µl)||---||In Stock||---|
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|Kit Includes||Quantity||Applications||Reactivity||Homology†||MW (kDa)||Isotype|
|Aldolase A (D73H4) Rabbit mAb #8060||40 µl||W||H, M, Mk||40||Rabbit IgG|
|Enolase-1 Antibody #3810||40 µl||W, IP||H, M, R, Mk||47||Rabbit|
|Enolase-2 (D20H2) Rabbit mAb #8171||40 µl||W, IP||H, M, R, Mk||47||Rabbit IgG|
|PDHK1 (C47H1) Rabbit mAb #3820||40 µl||W, IP||H, M, R, Mk||B||47||Rabbit IgG|
|PFKFB2 (D7G5R) Rabbit mAb #13045||40 µl||W, IP, IF-IC||H, Mk||55||Rabbit IgG|
|PFKFB3 (D7H4Q) Rabbit mAb #13123||40 µl||W||H, M, R, Mk||60||Rabbit IgG|
|PFKL Antibody #8175||40 µl||W||H, M, R, Mk||78||Rabbit|
|PGAM1 (D3J9T) Rabbit mAb #12098||40 µl||W||H, M, R, Hm, Mk||28||Rabbit IgG|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||Goat|
†Species predicted to react based on 100% sequence homology.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: H=Human, M=Mouse, Mk=Monkey, R=Rat, Hm=Hamster
Western blot analysis of extracts from various cell lines using PGAM1 (D3J9T) Rabbit mAb #12098.
Western blot analysis of extracts from LNCaP, Hep G2, and MCF7 cells using PFKFB2 (D7G5R) Rabbit mAb #13045 (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of extracts from various cell lines using PFKFB3 (D7H4Q) Rabbit mAb #13123.
Western blot analysis of extracts from various cell types using Enolase-1 Antibody #3810.
Western blot analysis of extracts from various cell types using PDHK1 (C47H1) Rabbit mAb #3820.
Western blot analysis of extracts from various cell lines using Aldolase A (D73H4) Rabbit mAb #8060.
Western blot analysis of extracts from SH-SY5Y, L18, and U-118 MG cells using Enolase-2 (D20H2) Rabbit mAb #8171.
The Glycolysis II Antibody Sampler Kit provides an economical means to investigate select enzymes involved in glycolysis. The kit contains enough primary antibody to perform four western blot experiments per primary antibody.
Specificity / Sensitivity
Each antibody recognizes endogenous total levels of the specified target protein independent of its modified state.
Source / Purification
Monoclonal antibodies are produced by immunizing animals with a synthetic peptides corresponding to residues surrounding Pro263 of human fructose bisphosphate aldolase A protein, the carboxy terminus of human enolase-2 protein, human PDHK1 protein, residues surrounding Pro454 within the fructose-2,6-biphosphatase region of human PFKFB2 protein, residues surrounding Leu456 of human PFKFB3 protein, and the carboxy terminus of human PGAM1 protein. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human enolase-1 and carboxy terminus of human PFKL protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.
Glycolysis is the metabolic process by which glucose is converted to pyruvate in a sequence of enzymatic steps. Phosphofructokinase (PFK) catalyzes the phosphorylation of fructose-6-phosphate in glycolysis (1). The bifunctional 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFK-2/FBPase or PFKFB) catalyzes the synthesis and degradation of fructose 2,6-bisphosphate and regulates its steady-state level. Four different PFKFB isoforms (PFKFB1, PFKFB2, PFKFB3, and PFKFB4) have been identified (2). Aldolase (fructose bisphosphate aldolase) is a glycolytic enzyme that catalyzes the conversion of fructose 1, 6-bisphosphate to 3-phosphoglyceraldehyde (3). Phosphoglycerate mutase (PGAM1) catalyzes the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis (4). Enolase is an important glycolytic enzyme involved in the interconversion of 2-phosphoglycerate to phosphoenolpyruvate. Mammalian enolase exists as three subunits: enolase-1 (α-enolase), enolase-2 (γ-enolase) and enolase-3 (β-enolase) that can form both homo- and heterodimers (5). Pyruvate dehydrogenase kinase (PDHK) phosphorylates PDH and inactivates it, whereas dephosphorylation of PDH is carried out by pyruvate dehydrogenase phosphatase to generate the active form (6).
- Mediavilla, D. et al. (2008) J Biochem 144, 235-44.
- Atsumi, T. et al. (2005) Diabetes 54, 3349-57.
- Castaldo, G. et al. (2000) Clin Chem 46, 901-6.
- Vander Heiden, M.G. et al. (2010) Science 329, 1492-9.
- Pancholi, V. (2001) Cell Mol Life Sci 58, 902-20.
- Wigfield, S.M. et al. (2008) Br J Cancer 98, 1975-84.
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* Product-specific protocol.
- 8337 Glycolysis Antibody Sampler Kit
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 9998 BSA
- 9999 Nonfat Dry Milk
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 9997 Tris Buffered Saline with Tween® 20 (TBST-10X)
- 7722 Blue Loading Buffer Pack
- 7723 Red Loading Buffer Pack
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7075 Anti-biotin, HRP-linked Antibody
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
Select rabbit monoclonal antibodies are developed, validated, and produced at CST using in part technology under license (granting certain rights including those under U.S. Patents No. 5,675,063 and in some instances 7,429,487) from Epitomics, Inc.