Product Pathways - Neuroscience
VAMP1 Antibody #13151
|13151S||100 µl (10 western blots)||---||In Stock||---|
|13151||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat||Endogenous||16||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Species predicted to react based on 100% sequence homology: Monkey.
Specificity / Sensitivity
VAMP1 Antibody recognizes endogenous levels of total VAMP1 protein. This antibody does not cross-react with VAMP2.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human VAMP1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc-tagged full length human VAMP1 (hVAMP1-Myc; +) or VAMP2 (hVAMP2-Myc; +), using VAMP1 Antibody (upper) and Myc-Tag (71D10) Rabbit mAb #2278 (lower).
Immunoprecipitation of VAMP1 from mouse brain using Normal Rabbit IgG #2729 (lane 2) or VAMP1 Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using VAMP1 Antibody.
Vesicle-associated membrane protein 1 (VAMP1), also called synaptobrevin 1, is part of the R-soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex (1). The SNARE complex is involved in calcium regulated vesicular transport and membrane fusion (2). While related protein VAMP2 exhibits a wider distribution and is more abundant in the brain, VAMP1 is the main isoform in specific brain regions including the subthalamus nucleus zona incerta (1), the ostral periolivary region, and the retina (3). In addition, VAMP1 is involved in neurotransmitter release at the neuromuscular junction (4) and in the release of bioactive peptides from cardiac myocytes (5).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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