Product Pathways - Protein Stability
SignalSilence® SYVN1 siRNA I #13204
|13204S||300 µl (3 nmol)||---||In Stock||---|
|13204||carrier free and custom formulation / quantity||email request|
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Species predicted to react based on 100% sequence homology: Monkey.
Western blot analysis of extracts from 293T cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® SYVN1 siRNA I (+), using SYVN1 Antibody #12925 (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). The SYVN1 Antibody confirms silencing of SYVN1 expression, while the GAPDH (D16H11) XP® Rabbit mAb is used as a loading control.
SignalSilence® SYVN1 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit SYVN1 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions For Use
CST recommends transfection with 100 nM SignalSilence® SYVN1 siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.
Each vial contains the equivalent of 100 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 μl per well.
Synoviolin-1 (SYVN1/HRD1) is a RING-type E3 ubiquitin-protein ligase and major component of the endoplasmic reticulum (ER) quality control system that is involved in the ubiquitin-dependent degradation of misfolded proteins (1). SYVN1 is a multispanning ER membrane protein whose expression is upregulated at the protein level under conditions that promote ER stress (1-4). Research studies have shown that SYVN1 is an anti-apoptotic factor that is implicated in the pathogenesis of arthropathy by promoting synovial hyperplasia (5). Furthermore, gene-targeting studies have demonstrated that SYVN1 expression is indispensable for embryogenesis (6).
- Kikkert, M. et al. (2004) J Biol Chem 279, 3525-34.
- Kaneko, M. et al. (2007) FEBS Lett 581, 5355-60.
- Yamamoto, K. et al. (2008) J Biochem 144, 477-86.
- Nadav, E. et al. (2003) Biochem Biophys Res Commun 303, 91-7.
- Amano, T. et al. (2003) Genes Dev 17, 2436-49.
- Yagishita, N. et al. (2005) J Biol Chem 280, 7909-16.
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For Research Use Only. Not For Use In Diagnostic Procedures.
SignalSilence® is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.