Product Pathways - Transcription Factors
ThPOK (D9V5T) Rabbit mAb #13205
|13205S||100 µl (10 western blots)||---||In Stock||---|
|13205||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse||Endogenous||75||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, F=Flow Cytometry
* Product-specific protocol.
Specificity / Sensitivity
ThPOK (D9V5T) Rabbit mAb recognizes endogenous levels of total ThPOK protein. This antibody cross-reacts with a 40 kDa protein of unknown origin.
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant human ThPOK protein.
Western blot analysis of mouse CD4+ T cells and mouse CD8+ T cells using ThPOK (D9V5T) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Flow cytometric analysis of mouse splenocytes using ThPOK (D9V5T) Rabbit mAb and co-stained with CD4-FITC and CD8-PE . CD8+ gated cells (blue) are negative for ThPOK and CD4+ cells (red) are ThPOK positive. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody.
The transcription factor Th-inducing POZ/Krüppel-like factor (ThPOK, ZBTB7B, cKROX, ZFP67) is a transcriptional repressor belonging to the POK/ZBTB family of lymphoid cell development regulators (1). ThPOK is best known as a signature CD4+ Th cell transcription factor that is upregulated during the differentiation of CD4+ Th but not CD8+ cytotoxic T cells (1). Expression of ThPOK in developing T cells represses expression of CD8 and cytotoxic T cell effector genes, and indirectly promotes expression of CD4 by antagonizing RUNX-mediated CD4 repression (2-4). ThPOK expression has also been observed in NKT cells and γδ T cells (5,6).
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