Product Pathways - Jak/Stat Pathway
Phospho-Stat4 (Tyr693) (D2E4) Rabbit mAb (PE Conjugate) #13223
|13223S||100 µl (50 tests)||---||In Stock||---|
|13223||carrier free and custom formulation / quantity||email request|
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Applications Key: F=Flow Cytometry
Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey, Pig.
Specificity / Sensitivity
Phospho-Stat4 (Tyr693) (D2E4) Rabbit mAb (PE Conjugate) recognizes endogenous levels of Stat4 protein only when phosphorylated at Tyr693.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr693 of human Stat4 protein.
This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-Stat4 (Tyr693) (D2E4) Rabbit mAb #4134.
The Jak-Stat signaling pathway is utilized by a large number of cytokines, growth factors, and hormones (1). Receptor-mediated tyrosine phosphorylation of Jak family members triggers phosphorylation of Stat proteins, resulting in their nuclear translocation, binding to specific DNA elements, and subsequent activation of transcription. The remarkable range and specificity of responses regulated by the Stats is determined, in part, by the tissue-specific expression of different cytokine receptors, Jaks, and Stats, as well as by the combinatorial coupling of various Stat members to different receptors (2). Stat4 is predominantly expressed in the spleen, thymus, and testis and has been most extensively investigated as the mediator of IL-12 responses (3-8). Activation of Stat4 is associated with phosphorylation at Tyr693 (9).
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- Visconti, R. et al. (2000) Blood 96, 1844-52.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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