Product Pathways - Tyrosine Kinase / Adaptors
LIMD1 Antibody #13245
|13245S||100 µl (10 western blots)||---||In Stock||---|
|13245||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
LIMD1 Antibody recognizes endogenous levels of total LIMD1 protein. This antibody cross-reacts with a protein of unknown origin at ~45 kDa.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human LIMD1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
LIM domain-containing protein 1 (LIMD1) is a putative tumor suppressor and adapter/scaffold protein that belongs to the Ajuba family of LIM domain containing proteins. LIM domain containing proteins mediate protein-protein interactions and typically contain a pair of distinct zinc finger domains (1). Research studies indicate that LIMD1 is involved in numerous cellular processes, including inhibition of E2F mediated transcription (2) and negative regulation of the Hippo pathway through influence on YAP phosphorylation state (3,4). Additional studies identify LIMD1 as a hypoxia regulator as it recruits the Von Hippel-Lindau (VHL) protein and the hydroxylase PHD1 to a protein complex that promotes initiation of HIF-1α ubiquitination and degradation (5). Research evidence supporting the role of LIMD1 as a tumor suppressor includes the down regulation of the protein in 80% of lung cancers (6), loss of LIMD1 expression in head and neck cancers (7), and altered subcellular localization in cases of breast cancer (8).
- Schmeichel, K.L. and Beckerle, M.C. (1994) Cell 79, 211-9.
- Sharp, T.V. et al. (2004) Proc Natl Acad Sci U S A 101, 16531-6.
- Sun, G. and Irvine, K.D. (2013) Sci Signal 6, ra81.
- Reddy, B.V. and Irvine, K.D. (2013) Dev Cell 24, 459-71.
- Foxler, D.E. et al. (2012) Nat Cell Biol 14, 201-8.
- Sharp, T.V. et al. (2008) Proc Natl Acad Sci U S A 105, 19932-7.
- Ghosh, S. et al. (2010) Mol Cancer 9, 58.
- Spendlove, I. et al. (2008) Int J Cancer 123, 2247-53.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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