Product Pathways - TGF-beta/Smad Signaling
Sara (D5X4F) Rabbit mAb #13285
|13285S||100 µl (10 western blots)||---||In Stock||---|
|13285||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human||Endogenous||190, 210||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
Sara (D5X4F) Rabbit mAb recognizes endogenous levels of total Sara protein. This antibody also recognizes a non-specific band of unknown origin at 50 kDa.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val488 of human Sara protein.
Western blot analysis of extracts from SH-SY5Y cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® Sara siRNA I (+) #13542, or SignalSilence® Sara siRNA II #13553 (+), using Sara (D5X4F) Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). The Sara (D5X4F) Rabbit mAb confirms silencing of Sara expression, while the α-Actinin (D6F6) XP® Rabbit mAb is used as a loading control.
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing full-length human Sara protein (hSara; +), using Sara (D5X4F) Rabbit mAb.
Western blot analysis of extracts from various cell lines using Sara (D5X4F) Rabbit mAb.
The Smad anchor for receptor activation (SARA, ZFYVE9) protein is an FYVE domain-containing protein originally identified as a regulator of TGF-β signaling (1). FYVE domains are zinc finger-like domains that bind to phosphatidylinositol 3-phosphate and are responsible for endosomal trafficking (2). While the role of Sara in TGF-β signaling has been questioned (3,4), early research studies demonstrate that Sara enhances TGF-β signaling by binding and recruiting non-activated Smad2 and Smad3 to the TGF-β receptor complex (1). Upon Smad2 activation, Sara dissociates from the complex while phosphorylated Smad2/3 translocates to the nucleus to bind to the common Smad, Smad4. Sara can also function as an anchor for the protein phosphatase 1 (PP1c) catalytic subunit, which is involved in the Smad7-mediated dephosphorylation of TGF-β type I receptor (5,6). Additional research studies show that expression of Sara plays a critical role in maintenance of the epithelial cell phenotype and that expression is regulated during the epithelial-to-mesenchymal transition (EMT) and fibrosis (7,8).
- Tsukazaki, T. et al. (1998) Cell 95, 779-91.
- Itoh, F. et al. (2002) Genes Cells 7, 321-31.
- Bakkebø, M. et al. (2012) FEBS Lett 586, 3367-72.
- Goto, D. et al. (2001) Biochem Biophys Res Commun 281, 1100-5.
- Bennett, D. and Alphey, L. (2002) Nat Genet 31, 419-23.
- Shi, W. et al. (2004) J Cell Biol 164, 291-300.
- Runyan, C.E. et al. (2009) J Biol Chem 284, 25181-9.
- Zhao, B.M. and Hoffmann, F.M. (2006) Mol Biol Cell 17, 3819-31.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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