Product Pathways - Metabolism
Thioredoxin 2 Antibody #13322
|13322S||100 µl (10 western blots)||---||In Stock||---|
|13322||carrier free and custom formulation / quantity||email request|
Already purchased this product? Write a Review.
|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||13||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
Thioredoxin 2 Antibody recognizes endogenous levels of total thioredoxin 2 protein. This antibody may cross-react with unidentified proteins from 60 kDa to 140 kDa.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human thioredoxin 2 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from various cell lines using Thioredoxin 2 Antibody.
Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing Myc-tagged full-length human thioredoxin 2 (hTRX2-Myc; +), using Thioredoxin 2 Antibody (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Thioredoxin is a small redox protein found in many eukaryotes and prokaryotes. A pair of cysteines within a highly conserved, active site sequence can be oxidized to form a disulfide bond that is then reduced by thioredoxin reductase (1). Multiple forms of thioredoxin have been identified, including cytosolic thioredoxin 1 (TRX1) and mitochondrial thioredoxin 2 (TRX2). Thioredoxin participates in many cellular processes including redox signaling, response to oxidative stress and protein reduction (1). A potential role of thioredoxin in human disorders such as cancer, aging and heart disease is currently under investigation (2). Thioredoxin can play a key role in cancer progression, as it acts as a negative regulator of the proapoptotic kinase ASK1 (3). Changes in thioredoxin expression have been associated with meningococcal septic shock and acute lung injury (4,5).
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 2429 Thioredoxin 1 (C63C6) Rabbit mAb
- 2285 Thioredoxin 1 Antibody (Human Specific)
- 2298 Thioredoxin 1 Antibody (Mouse/Rat Preferred)
- 1057 Thioredoxin 1 Blocking Peptide
- 3206 GPX1 Antibody
- 3187 NQO1 (A180) Mouse mAb
- 2770 SOD1 Antibody
- 13141 SOD2 (D3X8F) XP® Rabbit mAb
- 13194 SOD2 (D9V9C) Rabbit mAb
- 9997 Tris Buffered Saline with Tween® 20 (TBST-10X)
- 9998 BSA
- 9999 Nonfat Dry Milk
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
Anti-FLAG® is a registered trademark of Sigma-Aldrich Biotechnology.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.