Product Pathways - Lymphocyte Signaling
CD79A (D1X5C) XP® Rabbit mAb #13333
|13333S||100 µl (10 western blots)||---||In Stock||---|
|13333P||40 µl (4 western blots)||---||In Stock||---|
|13333||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry
* Product-specific protocol.
Specificity / Sensitivity
CD79A (D1X5C) XP® Rabbit mAb recognizes endogenous levels of total CD79A protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu81 of human CD79A protein.
Immunohistochemical analysis of paraffin-embedded human lymphoma using CD79A (D1X5C) XP® Rabbit mAb.
Western blot analysis of extracts from various cell lines using CD79A (D1X5C) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of extracts from Ramos cells, untreated (-) or PNGase F-treated (+), using CD79A (D1X5C) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using CD79A (D1X5C) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using CD79A (D1X5C) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Flow cytometric analysis of human whole blood lymphocytes using CD79A (D1X5C) XP® Rabbit mAb. Cells were co-stained with CD19-APC and CD3-PE antibodies to allow for CD79A analysis in B cells (green) and T cells (blue), respectively. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Antigen receptors found on the surface of B cells contain a heterodimeric signaling component composed of CD79A and CD79B, also known as Ig α and Ig β, respectively (1,2). Presence of this receptor complex is essential for B-cell development and function (3). Together these two proteins and the associated B cell receptor initiate intracellular signaling following antigen binding (4,5). An immunoreceptor tyrosine-based activation motif (ITAM) found in the CD79A intracellular region appears to be important for its function (6). Antigen binding precedes formation of the CD79A and CD79B heterodimer and subsequent activation of receptor associated kinases (7). Research has shown that CD79A is a marker for B-lineage lymphoblastic leukemia (8). Additionally, investigators have found that mutations in the CD79A (MB1) gene are associated with abnormally low levels of functional B cell receptors in some cases of chronic B cell lymphocytic leukemia (9).
- van Noesel, C.J. et al. (1991) J Immunol 146, 3881-8.
- Minegishi, Y. et al. (1999) J Clin Invest 104, 1115-21.
- Yu, L.M. and Chang, T.W. (1992) J Immunol 148, 633-7.
- Storch, B. et al. (2007) Eur J Immunol 37, 252-60.
- Mason, D.Y. et al. (1995) Blood 86, 1453-9.
- Luisiri, P. et al. (1996) J Biol Chem 271, 5158-63.
- Pike, K.A. et al. (2004) J Immunol 172, 2210-8.
- Astsaturov, I.A. et al. (1996) Leukemia 10, 769-73.
- Vuillier, F. et al. (2005) Blood 105, 2933-40.
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For Research Use Only. Not For Use In Diagnostic Procedures.
XP® is a trademark of Cell Signaling Technology, Inc.
Tween® is a registered trademark of ICI Americas, Inc.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.