Product Pathways - Protein Stability
UBE1L2/UBA6 Antibody #13386
|13386S||100 µl (10 western blots)||---||In Stock||---|
|13386||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||117||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Species predicted to react based on 100% sequence homology: Pig.
Specificity / Sensitivity
UBE1L2/UBA6 Antibody recognizes endogenous levels of total UBE1L2/UBA6 protein. This antibody does not cross-react with UBE1/UBA1 or UBE1L/UBA7 proteins.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys810 of human UBE1L2 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from various cell lines using UBE1L2/UBA6 Antibody.
Immunoprecipitation of UBE1L2/UBA6 from 293T cell extracts using Normal Rabbit IgG #2729 (lane 2) or UBE1L2/UBA6 Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using UBE1L2/UBA6 Antibody.
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human UBE1L2 protein (hUBE1L2-Myc/DDK; +), Myc/DDK-tagged full-length human UBE1 protein (hUBE1-Myc/DDK; +), or Myc/DDK-tagged full-length human UBE1L protein (hUBE1L-Myc/DDK; +), using UBE1L2/UBA6 Antibody (upper) or DYKDDDDK Tag Antibody #2368 (lower).
Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thioester complex with the ubiquitin-activating enzyme (E1). The activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, and then from E2 to ubiquitin ligase E3 for final delivery to the ε-amino group of the target protein lysine residue (1-3).
Ubiquitin-activating enzyme E1-like protein 2/Ubiquitin-like modifier-activating enzyme 6 (UBE1L2/UBA6) is ubiquitously expressed in human tissues and functions as an E1 enzyme related to UBE1/UBA1 (40% identity at the protein level). UBE1L2/UBA6 activates both ubiquitin and the ubiquitin-like protein FAT10 through a similar ATP dependent mechanism (4-6). Like other E1 protein family members, UBE1L2/UBA6 contains a conserved ATP-binding adenylation domain and an active site cysteine residue that are critical for enzymatic function (4,5). Research studies have demonstrated that UBE1L2/UBA6 expression is essential during the early stages of embryogenesis in mice (4). Furthermore, loss of neuronal UBE1L2/UBA6 expression promotes significant defects in neuronal structure and function, which contributes to a reduction in body weight and decreased postnatal viability (7).
- Ciechanover, A. (1998) EMBO J 17, 7151-60.
- Hochstrasser, M. (2000) Nat Cell Biol 2, E153-7.
- Hochstrasser, M. (2000) Science 289, 563-4.
- Chiu, Y.H. et al. (2007) Mol Cell 27, 1014-23.
- Pelzer, C. et al. (2007) J Biol Chem 282, 23010-4.
- Jin, J. et al. (2007) Nature 447, 1135-8.
- Lee, P.C. et al. (2013) Mol Cell 50, 172-84.
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