Product Pathways - Chromatin Regulation / Epigenetics
DNMT3L (E1Y7Q) Rabbit mAb (Mouse Specific) #13451
|13451S||100 µl (10 western blots)||---||In Stock||---|
|13451||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
DNMT3L (E1Y7Q) Rabbit mAb (Mouse Specific) recognizes endogenous levels of total DNMT3L protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val385 of mouse DNMT3L protein.
Western blot analysis of extracts from F9 and mES cells using DNMT3L (E1Y7Q) Rabbit mAb (Mouse Specific).
Western blot analysis of extracts from F9 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® DNMT3L siRNA I (Mouse Specific) #12307 (+), or SignalSilence® DNMT3L siRNA II (Mouse Specific) #12308 (+), using DNMT3L (E1Y7Q) Rabbit mAb (Mouse Specific) (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). The DNMT3L (E1Y7Q) Rabbit mAb (Mouse Specific) confirms silencing of DNMT3L expression, while the GAPDH (D16H11) XP® Rabbit mAb is used as a loading control.
Immunoprecipitation of DNMT3L from F9 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or DNMT3L (E1Y7Q) Rabbit mAb (Mouse Specific) (lane 3). Lane 1 is 10% input. Western blot analysis was performed using DNMT3L (E1Y7Q) Rabbit mAb (Mouse Specific).
Methylation of DNA at cytosine residues in mammalian cells is a heritable, epigenetic modification that is critical for proper regulation of gene expression, genomic imprinting, and development (1,2). Three families of mammalian DNA methyltransferases have been identified: DNMT1, DNMT2, and DNMT3 (1,2). DNMT1 is constitutively expressed in proliferating cells and functions as a maintenance methyltransferase, transferring proper methylation patterns to newly synthesized DNA during replication. DNMT3A and DNMT3B are strongly expressed in embryonic stem cells with reduced expression in adult somatic tissues. DNMT3A and DNMT3B function as de novo methyltransferases that methylate previously unmethylated regions of DNA. DNMT2 is expressed at low levels in adult somatic tissues and its inactivation affects neither de novo nor maintenance DNA methylation.
DNMT3L is a catalytically inactive regulatory factor for the DNMT3A and DNMT3B de novo methyltransferases that is expressed at low levels in embryonic stem cells, testis, ovaries, and thymus (1,2). These de novo methyltransferases consist of a heterotetrameric complex containing two molecules of DNMT3L, and either two molecules of DNMT3A or DNMT3B (3). DNMT3L contains an amino-terminal ATRX-DNMT3-DNMT3L (ADD) domain and a carboxy-terminal methyltransferase-like domain (4-7). The methyltransferase-like domain binds to DNMT3A and DNMT3B to stimulate catalytic activity by increasing the binding of S-adenosylmethionine and DNA (4,5). The ADD domain recruits the methyltransferase complex to transcriptionally inactive regions of the genome by binding to unmethylated histone H3 Lys4 (6,7).
- Hermann, A. et al. (2004) Cell Mol Life Sci 61, 2571-87.
- Turek-Plewa, J. and Jagodziński, P.P. (2005) Cell Mol Biol Lett 10, 631-47.
- Jia, D. et al. (2007) Nature 449, 248-51.
- Holz-Schietinger, C. and Reich, N.O. (2010) J Biol Chem 285, 29091-100.
- Suetake, I. et al. (2004) J Biol Chem 279, 27816-23.
- Ooi, S.K. et al. (2007) Nature 448, 714-7.
- Otani, J. et al. (2009) EMBO Rep 10, 1235-41.
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For Research Use Only. Not For Use In Diagnostic Procedures.
XP® is a trademark of Cell Signaling Technology, Inc.
Tween® is a registered trademark of ICI Americas, Inc.
SignalSilence® is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.