Product Pathways - Chromatin Regulation / Epigenetics
SNF2H Antibody #13453
|13453S||100 µl (10 western blots)||---||In Stock||---|
|13453||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Species predicted to react based on 100% sequence homology: Bovine, Dog, Pig, Guinea Pig, Horse.
Specificity / Sensitivity
SNF2H Antibody recognizes endogenous levels of total SNF2H (SMARCA5) protein. This antibody does not cross-react with SMARCA1 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala65 of human SNF2H (SMARCA5) protein. Antibodies are purified by protein A and peptide affinity chromatography.
Sucrose nonfermenting 2 homolog (SNF2H, SMARCA5) is one of two orthologs of the ISWI (imitation switch) ATPases encoded by the mammalian genome (1). SNF2H is part of the SNF2 family of chromatin remodeling factors that use ATP hydrolysis to catalyze biochemical reactions in several mammalian chromatin-remodeling complexes, including ACF1, RSF1, CHRAC, NoRC, WSTF, and WCRF180 (3). Research studies show that SNF2H is crucial for chromatin organization, DNA damage response, and differentiation (1-7). The SNF2H helicase facilitates DNA damage repair by actively moving nucleosomes for DNA damage response (DDR) proteins to effectively associate with damaged regions (3). Additional studies show that repair of double stranded breaks (DSBs) significantly decreases in the absence of SNF2H (3), and these cells become highly sensitive to DNA damage caused by x-rays and chemical treatments inducing DSBs (4,5).
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- Helfricht, A. et al. (2013) Cell Cycle 12, 3070-82.
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- Mueller, A.C. et al. (2013) Oncogene 32, 1164-72.
- Lan, L. et al. (2010) Mol Cell 40, 976-87.
- Smeenk, G. et al. (2013) J Cell Sci 126, 889-903.
- Chioda, M. et al. (2010) Development 137, 3513-22.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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