Product Pathways - Chromatin Regulation / Epigenetics
CHD1L (E1I8C) Rabbit mAb #13460
|13460S||100 µl (10 western blots)||---||In Stock||---|
|13460||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
CHD1L (E1I8C) Rabbit mAb recognizes endogenous levels of total CHD1L protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly231 of human CHD1L protein.
Western blot analysis of extracts from various cell lines using CHD1L (E1I8C) Rabbit mAb.
Chromodomain-helicase-DNA-binding domain 1 like (CHD1L) is a DNA helicase and member of the SNF2 subfamily of ATP dependent chromatin remodeling enzymes (1). Unlike other CHD chromatin remodeling proteins, CHD1L lacks a methylated histone binding chromodomain but does contain a macro domain that binds poly-ADP ribosylated (PARylated) targets (1,2). Following genotoxic stress, CHD1L interacts with PARylated PARP1 and is recruited to sites of DNA damage to facilitate DNA repair (3,4). The CHD1L protein is often over expressed in metastatic hepatocellular carcinoma (HCC) and the corresponding CHD1L gene is located in a region that is frequently amplified in cases of HCC (5-7). Research studies indicate that CHD1L over expression may lead to over relaxation of chromatin and exposing the underlying DNA to genotoxic stress (1). CHD1L can regulate expression of genes that promote tumor cell proliferation, migration and metastasis, providing another mechanism where CHD1L may promote hepatocellular carcinoma progression and metastasis (6,8). Additional research studies suggest that over expression of CHD1L may be involved in the progression of bladder, colon and ovary cancer (9-11).
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