Product Pathways - Cytoskeletal Signaling
PKG-1α (D10G2) Rabbit mAb #13511
|13511S||100 µl (10 western blots)||---||In Stock||---|
|13511||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat||Endogenous||78||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
PKG-1α (D10G2) Rabbit mAb recognizes endogenous levels of total PKG-1α protein. This antibody does not cross-react with PKG-1β.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human PKG-1α protein.
Western blot analysis of extracts from COS-7 cells, mock transfected (-) or transfected with constructs expressing full-length human PKG-1α (hPKG-1α; +) or PKG-1β (hPKG-1β; +), using PKG-1α (D10G2) Rabbit mAb (upper) or PKG-1 (C8A4) Rabbit mAb #3248 (lower).
Cyclic GMP-dependent kinases (cGK/PKG) belong to the AGC family of serine/threonine protein kinases. In mammals, two genes encode PKG-1 and PKG-2. Alternative PKG-1 splicing yields α and β isoforms, which display tissue-specific expression patterns in humans (1). All PKG family members are activated by increased cellular cGMP, which binds to the enzyme's regulatory domain inducing a conformational change and leading to enzyme activation. cGMP levels are increased through the activation of guanylyl cyclases, a process known to occur in part through nitric oxide (NO) signaling (2).
In addition to well established roles in platelet activation and smooth muscle relaxation (3), PKG signaling is important in many biological processes including cardiac contractility, axon guidance, bone growth, contraction of intestinal smooth muscle, and erectile dysfunction (4).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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