Product Pathways - Cytoskeletal Signaling
SSH1 (E1K3W) Rabbit mAb #13578
|13578S||100 µl (10 western blots)||---||In Stock||---|
|13578||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat||Endogenous||140||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
SSH1 (E1K3W) Rabbit mAb recognizes endogenous levels of total SSH1 protein. Based on the absence of sequence homology, this antibody is not expected to recognize SSH2 or SSH3.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro1018 of human SSH1 protein.
Western blot analysis of extracts from various cell lines using SSH1 (E1K3W) Rabbit mAb.
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human SSH1 protein (hSSH1-Myc/DDK; +), using SSH1 (E1K3W) Rabbit mAb (upper) or Myc-Tag (71D10) Rabbit mAb #2278 (lower).
Immunoprecipitation of SSH1 from Jurkat cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or SSH1 (E1K3W) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using SSH1 (E1K3W) Rabbit mAb.
Cofilin is an evolutionarily conserved, actin-binding protein that severs actin filaments during processes that rely on actin filament dynamics, including cytokinesis, cell migration, invasion, and neuronal development. Actin severing and filament depolymerization are regulated through the controlled cycling of cofilin between the phosphorylated and dephosphorylated forms (1). The kinases LIMK and TESK inactivate cofilin by phosphorylating it at Ser3 (2,3). The slingshot homologs (SSH1, SSH2 and SSH3) and chronophin/PDXP phosphatases remove phosphate from cofilin at Ser3, enabling cofilin binding to actin and filament depolymerization (3). LIMK and SSH1 regulate cofilin activity downstream of neuregulin signaling in Schwann cells (4).
Slingshot homolog 1 (SSH1) can also dephosphorylate LIMK kinases, suppressing LIMK phosphorylation of cofilin (5). In addition, SSH1 modulates actin dynamics by stabilizing F-actin and promoting actin bundling independent of its cofilin phosphatase activity (6). SSH1 activity is regulated by phosphorylation and protein-protein interaction through various signaling pathways (1). Binding of SSH1 to F-actin stimulates its cofilin phosphatase activity (7).
- Mizuno, K. (2013) Cell Signal 25, 457-69.
- Toshima, J. et al. (2001) J Biol Chem 276, 31449-58.
- Huang, T.Y. et al. (2006) Curr Opin Cell Biol 18, 26-31.
- Sparrow, N. et al. (2012) J Neurosci 32, 5284-97.
- Soosairajah, J. et al. (2005) EMBO J 24, 473-86.
- Kurita, S. et al. (2007) Genes Cells 12, 663-76.
- Kurita, S. et al. (2008) J Biol Chem 283, 32542-52.
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