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Commit: 33ae406c5df2bbc7a7d3c17605228fbeff8ea416
XML generation date: 2026-04-23 13:33:38.755
Product last modified at: 2026-04-15T08:00:10.264Z
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PDP - Template Name: Monoclonal Antibody (Alexa Fluor Conjugate)
PDP - Template ID: *******c8ce56b
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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

EBF1 (E2I3Q) Rabbit Monoclonal Antibody (Alexa Fluor® 647 Conjugate) #17155

Filter:
  • F

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Rabbit IgG
    Application Key:
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Description

    This Cell Signaling Technology® antibody is conjugated to Alexa Fluor® 647 fluorescent dye under optimal conditions. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated EBF1 (E2I3Q) Rabbit Monoclonal Antibody #57794.

    Fluorescent Properties

    • ← Excitation: 650 nm ← Emission: 668 nm

    Product Usage Information

    Application Dilution
    Flow Cytometry (Fixed/Permeabilized) 1:100

    Storage

    Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

    Protocol

    Specificity / Sensitivity

    EBF1 (E2I3Q) Rabbit Monoclonal Antibody (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of total EBF1 protein. This antibody may cross-react with EBF2 and EBF3.

    Species Reactivity:

    Human

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly500 of human EBF1 protein.

    Background

    Early B-cell factor 1 (EBF1), also referred to as COE1, is a pioneering transcription factor that is crucial for defining B cell lineage. In hematopoiesis, EBF1 promotes the expression of genes that specify B cell lineage commitment, such as FoxO1, PAX5, and IRF-4, while also repressing the expression of transcription factor genes that promote alternate lineages (1,2). EBF1 has also been reported to play a role in olfactory neuronal cell, adipocyte, and early osteoclast differentiation (3-6). Due to the crucial role of EBF1 in B cell development, mutations in EBF1 can lead to the development of B-cell acute lymphoblastic leukemia (B-ALL) (7,8). EBF1 expression is also suppressed in some solid tumor cancers, including genomic loss in breast cancer and downregulation in pancreatic ductal adenocarcinomas (9,10). Lower expression levels of EBF1 also correlate with lower survival rates in patients with cholangiocarcinoma and colorectal carcinoma (CRC), and induced overexpression of EBF1 in CRC cell lines induces cell cycle arrest and apoptosis in vivo and in vitro (11,12).

    Alternate Names

    COE1; Collier, Olf and EBF transcription factor 1; early B cell factor 1; Early B-cell factor; early B-cell factor 1; EBF; EBF transcription factor 1; EBF1; FLJ39389; FLJ41763; O/E-1; OE-1; OLF1; olfactory neuronal transcription factor 1; Transcription factor COE1

    For Research Use Only. Not for Use in Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    Alexa Fluor is a registered trademark of Life Technologies Corporation.
    This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.