Revision 1

#18582

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IP

Reactivity:
H M

Sensitivity:
Endogenous

MW (kDa):
55

Source/Isotype:
Rabbit IgG

UniProt ID:
#Q14318

Entrez-Gene Id:
23770

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity/Sensitivity

FKBP8 (E6F3T) Rabbit Monoclonal Antibody recognizes endogenous levels of total FKBP8 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu72 of human FKBP8 protein.

Background

FKBP8, also known as FKBP38, is a member of the FK506-binding protein (FKBP) family that interacts with the immunosuppressive drug FK506 through a conserved peptidyl-prolyl cis-trans isomerase (PPIase) domain (1,2). In contrast to other FKBP family members, the PPIase activity of FKBP8 is dependent on Ca2+-signaling through a Ca2+-calmodulin binding domain (3). Loss of FKBP8 leads to defects in neural tube and eye development (4,5). FKBP family members generally function as molecular chaperones interacting with distinct proteins (2). FKBP8 is predominantly localized to the endoplasmic reticulum and the mitochondrial outer membrane and has been found to interact with proteins controlling apoptosis, including Bcl-2 and Bcl-xL (6). In addition, FKBP8 was found to promote autophagy through association with Beclin-1, a key component for activation of the VPS34 lipid kinase required for initiation of autophagy (7). FKBP8 also promotes autophagy by inhibiting mTOR, a kinase that plays a key role in cell growth (8). FKBP8 can also play a role in the clearance of damaged mitochondria through mitophagy. It contains an amino-terminal LC3-interacting region (LIR) motif that binds strongly to LC3A and recruits lipidated LC3A to damaged mitochondria to induce Parkin-independent mitophagy and promote mitochondrial fragmentation (9,10).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting IP: Immunoprecipitation

Cross-Reactivity Key

H: Human M: Mouse

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of extracts from various cell lines using FKBP8 (E6F3T) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Low expression of FKBP8 protein in NCI-H520 cells is consistent with the predicted expression pattern.
Western Blotting Image 1: FKBP8 (E6F3T) Rabbit Monoclonal Antibody
Western blot analysis of extracts from 293T cells, mock transfected (-) or transiently transfected with a construct expressing Myc/DDK-tagged full-length human FKBP8 protein (hFKBP8-Myc/DDK; +), using FKBP8 (E6F3T) Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Western Blotting Image 2: FKBP8 (E6F3T) Rabbit Monoclonal Antibody
Immunoprecipitation of FKBP8 protein from A-673 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is FKBP8 (E6F3T) Rabbit mAb. Western blot analysis was performed using FKBP8 (E6F3T) Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used as a secondary antibody.
Immunoprecipitation Image 1: FKBP8 (E6F3T) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.