Product Pathways - Apoptosis
Cleaved Lamin A (Asp230) Antibody #2031
|2031S||100 µl (10 western blots)||---||In Stock||---|
|2031||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat||Endogenous||45, 50||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
Cleaved Lamin A (Asp230) Antibody detects endogenous levels of the large fragment of lamin A (and lamin C isotype) resulting from cleavage at aspartic acid 230. The antibody does not recognize full length lamin A.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to carboxy-terminal residues surrounding Asp230 in human lamin A. Antibodies are purified by protein A and peptide affinity chromatography.
Lamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control, DNA replication, and chromatin organization (1-3). Lamin A/C is cleaved by caspase-6 and serves as a marker for caspase-6 activation. During apoptosis, lamin A/C is specifically cleaved into a large (41-50 kDa) and a small (28 kDa) fragment (3,4). The cleavage of lamins results in nuclear dysregulation and cell death (5,6).
(This product is sold under license from Promega Corp., U.S. Patent No. 6,350,452.)
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- Mintzer, R. et al. (2012) PLoS One 7, e30376. Applications: Western Blotting.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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