Product Pathways - Cytoskeletal Signaling
MCF2/Dbl Antibody #2089
|2089S||100 µl (10 western blots)||---||In Stock||---|
|2089||carrier free and custom formulation / quantity||email request|
Already purchased this product? Write a Review.
|W||1:1000||Human, Mouse, Rat||Endogenous||100||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
MCF2/Dbl Antibody recognizes endogenous levels of total MCF2/Dbl protein. Based on amino acid sequence homology, the antibody is expected to recognize splice variants 1-4 of human MCF2/Dbl. The antibody is not expected to recognize the onco-Dbl protein formed through amino-terminal truncation.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of human MCF2/Dbl. Antibodies are purified using protein A and peptide affinity chromatography.
The MCF2/Dbl proto-oncogene product is the founding member of the Dbl family of Rho guanine nucleotide exchange factors (GEFs) that are characterized by their Dbl homology (DH) domain (1). GEFs stimulate the formation of the active, GTP-bound form of small GTPases such as Rho, Rac and Cdc42, signaling to various downstream molecules and regulating diverse cell functions. While the overexpressed, full-length Dbl gene has transforming activity (2), mutations resulting in truncated Dbl cause the protein to become highly oncogenic. This truncated form of Dbl, which lacks the amino-terminal 497 amino acids, has constitutive GEF activity (3) and is more stable than the full-length variant (4), allowing for increased signaling to downstream effector molecules.
Dbl interacts with ezrin, a member of the ezrin/radixin/moesin (ERM) family of proteins that links the plasma membrane to the actin cytoskeleton. Dbl interacts with ezrin in lipid microdomains, which leads to Cdc42 activation and the regulation of processes such as filopodia formation and cell polarity (5,6). Dbl localization and biological activities are regulated in part by phosphatidylinositol 3-kinase (PI3K) (7). Dbl is also involved in cell survival and inhibits apoptosis through induction of Akt phosphorylation at Thr308 (8).
- Zheng, Y. (2001) Trends Biochem Sci 26, 724-32.
- Ron, D. et al. (1988) EMBO J 7, 2465-73.
- Hart, M.J. et al. (1991) Nature 354, 311-4.
- Kamynina, E. et al. (2007) Mol Cell Biol 27, 1809-22.
- Batchelor, C.L. et al. (2007) Cell Cycle 6, 353-63.
- Prag, S. et al. (2007) Mol Biol Cell 18, 2935-48.
- Vanni, C. et al. (2006) Cell Cycle 5, 2657-65.
- Morley, S. et al. (2007) Cell Signal 19, 211-8.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 2466 Cdc42 (11A11) Rabbit mAb
- 2461 Phospho-Rac1/cdc42 (Ser71) Antibody
- 2117 RhoA (67B9) Rabbit mAb
- 2098 RhoB Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.