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#24029

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
IF-F

Reactivity:
M

Sensitivity:
Endogenous

Source/Isotype:
Rabbit IgG

UniProt ID:
#Q61327

Entrez-Gene Id:
13162

Product Usage Information

Application Dilution
Immunofluorescence (Frozen) 1:100 - 1:400

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity/Sensitivity

Dopamine Transporter/DAT (E3U7R) Rabbit Monoclonal Antibody recognizes endogenous levels of total dopamine transporter/DAT protein. Non-specific labeling of mouse colon may be observed by immunofluorescence.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro26 of mouse dopamine transporter/DAT protein.

Background

Dopamine is a neurotransmitter that plays important roles in the brain, particularly in dopamine pathways that control the motivational component of reward-motivated behavior. These behavioral outputs are generated by the basal ganglia via its interaction with multiple brain areas that modulate sensorimotor, emotional, and cognitive information (1). The spatiotemporal dynamics of dopamine signaling are regulated by the dopamine transporter (DAT), a presynaptic transmembrane protein that drives the reuptake of dopamine released into the synaptic cleft. This process occurs in a sodium- and chloride-dependent manner, with DAT transporting two sodium cations down their concentration gradient, along with one chloride anion and the dopamine substrate. DAT is a member of the neurotransmitter sodium symporter (NSS) family, which also includes serotonin, norepinephrine, glycine, and γ-aminobutyric acid (GABA) transporters. The NSS family is part of the larger solute carrier 6 (SLC6) family of proteins, which in addition to neurotransmitters, also include transporters of amino acids, osmolytes, and energy metabolites (2-5). Mutations in the DAT gene, SLC6A3, have been linked to Parkinson’s disease (PD), infantile parkinsonism-dystonia (IPD), autism spectrum disorder (ASD), attention deficit hyperactivity disorder (ADHD), major depressive disorder (MDD), and bipolar disorder (BP) (5-8). In addition to its role in the brain, DAT is expressed by lymphocytes and monocytes in the periphery, where it modulates immune function. Altered expression levels of DAT in the periphery is also a suggested biomarker for some cancers (9-12).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

IF-F: Immunofluorescence (Frozen)

Cross-Reactivity Key

M: Mouse

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.