Product Pathways - Protein Stability
PSA/KLK3 (D11E1) XP® Rabbit mAb #2475
|2475S||100 µl (10 western blots)||---||In Stock||---|
|2475P||40 µl (4 western blots)||---||In Stock||---|
|2475||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin)
Specificity / Sensitivity
PSA/KLK3 (D11E1) XP® Rabbit mAb recognizes endogenous levels of total PSA/KLK3 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val72 of human PSA/KLK3 protein.
Western blot analysis of extracts from human sperm and LNCaP and DU 145 cells using PSA/KLK3 (D11E1) XP® Rabbit mAb. DU 145 cells are negative as expected.
Immunohistochemical analysis of paraffin-embedded cell pellets, LNCaP (left) or DU 145 (right), using PSA/KLK3 (D11E1) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using PSA/KLK3 (D11E1) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Kallikrein 3 (KLK3), also known as Prostate Specific Antigen (PSA), is a member of the glandular kallikrein subfamily of serine proteases (1). It is produced by prostate epithelial cells and is secreted into prostatic ducts. Upon cleavage of 7 N-terminal amino acids (2), it is activated to liquefy semen in the seminal coagulum. Although PSA/KLK3 is produced in healthy individuals, investigators have found abnormally high levels in the blood of men with advanced prostate cancer (3,4).
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For Research Use Only. Not For Use In Diagnostic Procedures.
XP® is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.