Product Pathways - Metabolism
TORC1/CRTC1 Antibody #2501
|2501S||100 µl (10 western blots)||---||In Stock||---|
|2501||carrier free and custom formulation / quantity||email request|
Already purchased this product? Write a Review.
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Species predicted to react based on 100% sequence homology: Human.
Specificity / Sensitivity
TORC1/CRTC1 Antibody recognizes endogenous levels of total TORC1 (CRTC1) protein. This antibody does not cross-react with TORC2 and TORC3 proteins.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly296 of human TORC1 (CRTC1) protein. Antibodies are purified by protein A and peptide affinity chromatography.
Glucose homeostasis is regulated by hormones and cellular energy status. Elevations of blood glucose during feeding stimulate insulin release from pancreatic β-cells through a glucose sensing pathway. Feeding also stimulates release of gut hormones such as glucagon-like peptide-1 (GLP-1), which further induces insulin release, inhibits glucagon release and promotes β-cell viability. CREB-dependent transcription likely plays a role in both glucose sensing and GLP-1 signaling (1). The protein CRTC2 (CREB-regulated transcription coactivator 2)/TORC2 (transducer of regulated CREB activity 2) functions as a CREB co-activator (2,3) and is implicated in mediating the effects of these two pathways (4). In quiescent cells, CRTC2/TORC2 is phosphorylated at Ser171 and becomes sequestered in the cytoplasm via an interaction with 14-3-3 proteins. Glucose and gut hormones lead to the dephosphorylation of CRTC2/TORC2 and its dissociation from 14-3-3 proteins. Dephosphorylated CRTC2/TORC2 enters the nucleus to promote CREB-dependent transcription. CRTC2/TORC2 plays a key role in the regulation of hepatic gluconeogenic gene transcription in response to hormonal and energy signals during fasting (5).
CRTC2/TORC2-related proteins CRTC1/TORC1 and CRTC3/TORC3 also act as CREB co-activators (2,3). CRTC1/TORC1, CRTC2/TORC2 and CRTC3/TORC3 associate with the HTLV Tax protein to promote Tax-dependent transcription of HTLV-1 long terminal repeats (6,7). CRTC1/TORC1 is highly phosphorylated at Ser151 in mouse hypothalamic cells under basal conditions (8). When these cells are exposed to cAMP or a calcium activator, CRTC1/TORC1 is dephosphorylated and translocates into the nucleus (8). CRTC1/TORC1 is essential for energy balance and fertility (8).
- Hinke, S.A. et al. (2004) J Physiol 558, 369-80.
- Conkright, M.D. et al. (2003) Mol Cell 12, 413-23.
- Iourgenko, V. et al. (2003) Proc Natl Acad Sci U S A 100, 12147-52.
- Screaton, R.A. et al. (2004) Cell 119, 61-74.
- Koo, S.H. et al. (2005) Nature 437, 1109-11.
- Koga, H. et al. (2004) J Biol Chem 279, 52978-83.
- Siu, Y.T. et al. (2006) J Virol 80, 7052-9.
- Altarejos, J.Y. et al. (2008) Nat Med 14, 1112-7.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 2587 TORC1/CRTC1 (C71D11) Rabbit mAb
- 3826 TORC2/CRTC2 Antibody
- 2768 TORC3/CRTC3 Antibody
- 9191 Phospho-CREB (Ser133) Antibody
- 9198 Phospho-CREB (Ser133) (87G3) Rabbit mAb
- 9196 Phospho-CREB (Ser133) (1B6) Mouse mAb
- 9197 CREB (48H2) Rabbit mAb
- 9104 CREB (86B10) Mouse mAb
- 9193 CREB Control Cell Extracts
- 1090 Phospho-CREB (Ser133) Blocking Peptide
- 9190 PhosphoPlus® CREB (Ser133) Antibody Kit
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 2720 TORC3/CRTC3 (C35G4) Rabbit mAb
For Research Use Only. Not For Use In Diagnostic Procedures.
Tween® is a registered trademark of ICI Americas, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.