Revision 1

#30718

Store at -20C

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IHC-P

Reactivity:
Vir

Sensitivity:
Endogenous

MW (kDa):
43

Source/Isotype:
Rabbit IgG

UniProt ID:
#P03418

Entrez-Gene Id:
1494470

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunohistochemistry (Paraffin) 1:250 - 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity/Sensitivity

Respiratory Syncytial Virus (RSV) Nucleoprotein (N) (F5S9J) Rabbit Monoclonal Antibody recognizes endogenous levels of total RSV nucleoprotein in RSV-infected cells and tissue. Based on sequence homology, this antibody will broadly detect RSV nucleoprotein subtypes and species variants.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro341 of human respiratory syncytial virus A (strain A2) nucleoprotein (UniProt #P03418).

Background

The respiratory syncytial virus (RSV), also known as human orthopneumovirus, is a negative-sense, single-stranded RNA virus that causes serious acute respiratory disease, primarily in infants and older adults. The RSV genome consists of 10 genes encoding 11 proteins. NS1 and NS2 are nonstructural proteins. Structural proteins are matrix (M), small hydrophobic (SH), attachment glycoprotein (G), fusion glycoprotein (F), transcriptional processivity factor (M2-1), RNA regulatory factor (M2-2), nucleoprotein (N), phosphoprotein (P), and large polymerase (L). The nucleocapsid is surrounded by a host-derived lipid envelope with protruding viral glycoproteins to form the virion (1,2). N coats and stabilizes the viral RNA, and is required for replication (3). Therapeutics targeting several viral proteins are in development, including monoclonal antibodies targeting F and G proteins, and small-molecule inhibitors targeting N and L proteins (4).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting IHC-P: Immunohistochemistry (Paraffin)

Cross-Reactivity Key

Vir: Virus

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of extracts from 293T cells, mock transfected (lane 1) or transfected with a construct expressing human respiratory syncytial virus A (HRSVA) nucleoprotein (lane 2), using Respiratory Syncytial Virus (RSV) Nucleoprotein (N) (F5S9J) Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Western Blotting Image 1: Respiratory Syncytial Virus (RSV) Nucleoprotein (N) (F5S9J) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded rat lung, control (left) or RSV-infected (right), using Respiratory Syncytial Virus (RSV) Nucleoprotein (N) (F5S9J) Rabbit mAb. Control and RSV-infected rat lung tissues were generously provided by Dr. Nicholas Crossland, National Emerging Infectious Diseases Laboratories, Boston University, Boston, MA.
Immunohistochemistry Image 1: Respiratory Syncytial Virus (RSV) Nucleoprotein (N) (F5S9J) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded RSV-infected rat lung using Respiratory Syncytial Virus (RSV) Nucleoprotein (N) (F5S9J) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right). RSV-infected rat lung tissues were generously provided by Dr. Nicholas Crossland, National Emerging Infectious Diseases Laboratories, Boston University, Boston, MA.
Immunohistochemistry Image 2: Respiratory Syncytial Virus (RSV) Nucleoprotein (N) (F5S9J) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded RSV-infected Hep2 cell pellet (left, positive) or HeLa cell pellet (right, negative) using Respiratory Syncytial Virus (RSV) Nucleoprotein (N) (F5S9J) Rabbit mAb. RSV-infected Hep2 cell pellet was generously provided by Dr. Nicholas Crossland, National Emerging Infectious Diseases Laboratories, Boston University, Boston, MA.
Immunohistochemistry Image 3: Respiratory Syncytial Virus (RSV) Nucleoprotein (N) (F5S9J) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded 293T cell pellet, untransfected (left) or Nucleoprotein (HRSVA) pneumovirus transfected (right), using Respiratory Syncytial Virus (RSV) Nucleoprotein (N) (F5S9J) Rabbit mAb.
Immunohistochemistry Image 4: Respiratory Syncytial Virus (RSV) Nucleoprotein (N) (F5S9J) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.