Product Pathways - MAPK Signaling
Phospho-MAPKAPK-2 (Thr222) (9A7) Rabbit mAb #3316
|3316S||100 µl (10 western blots)||---||In Stock||---|
|3316P||40 µl (4 western blots)||---||In Stock||---|
|3316||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||49||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
Phospho-MAPKAPK-2 (Thr222) (9A7) Rabbit mAb detects endogenous levels of MAPKAPK-2 protein only when phosphorylated at Thr222.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr222 of human MAPKAPK-2.
Western blot analysis of extracts from NIH/3T3 cells, untreated (-) or UV- and TPA-treated (+), using Phospho-MAPKAPK-2 (Thr222) (9A7) Rabbit mAb (upper) or MAPKAPK-2 Antibody #3042 (lower).
In response to cytokines, stress, and chemotactic factors, MAP kinase-activated protein kinase 2 (MAPKAPK-2) is rapidly phosphorylated and activated. It has been shown that MAPKAPK-2 is a direct target of p38 MAPK (1). Multiple residues of MAPKAPK-2 are phosphorylated in vivo in response to stress. However, only four residues (Thr25, Thr222, Ser272, and Thr334) are phosphorylated by p38 MAPK in an in vitro kinase assay (2). Phosphorylation at Thr222, Ser272, and Thr334 appears to be essential for the activity of MAPKAPK-2 (2). Thr25 is phosphorylated by p42 MAPK in vitro, but is not required for the activation of MAPKAPK-2 (2).
- Son, J.K. et al. (2010) Cell Death Differ 17, 1288-301. Applications: Western Blotting.
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
This antibody is developed, validated, and produced by CST using in part technology under license (granting certain rights including those under U.S. Patents No. 5,675,063 and 7,429,487) from Epitomics, Inc.