Product Pathways - NF-kB Signaling
STING Antibody #3337
|3337S||100 µl (10 western blots)||---||In Stock||---|
|3337||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
STING Antibody recognizes endogenous levels of total STING protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu325 of human STING protein. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from 293T cells, mock transfected (-), transfected with a cDNA expression construct encoding human STING (hSTING; +), or transfected with a cDNA expression constructed encoding mouse STING (mSTING; +), using STING Antibody.
Stimulator of interferon genes (STING, TMEM173, MITA) is a transmembrane adaptor protein that is a critical component of the cellular innate immune response to pathogenic cytoplasmic DNA (1,2). STING is a ubiquitously expressed protein found predominantly in the ER (1). The enzyme cGAMP synthase (cGAS) produces the second messenger cyclic-GMP-AMP (cGAMP) from cytoplasmic DNA (3,4). cGAMP binds and activates STING (3,4). In addition, detection of cytoplasmic DNA by nucleic acid sensors, including DDX41 or IFI16, results in STING activation (5,6). Following activation, STING translocates with TBK1 to perinuclear endosomes (7). The TBK1 kinase phosphorylates and activates interferon regulatory factors (IRFs) and NF-κB, which leads to the induction of type I interferon and other immune response genes (1,2,7).
- Ishikawa, H. and Barber, G.N. (2008) Nature 455, 674-8.
- Zhong, B. et al. (2008) Immunity 29, 538-50.
- Sun, L. et al. (2013) Science 339, 786-91.
- Wu, J. et al. (2013) Science 339, 826-30.
- Zhang, Z. et al. (2011) Nat Immunol 12, 959-65.
- Unterholzner, L. et al. (2010) Nat Immunol 11, 997-1004.
- Ishikawa, H. et al. (2009) Nature 461, 788-92.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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