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Plakophilin 1 (19F10) Mouse Monoclonal Antibody

Plakophilin 1 (19F10) Mouse Monoclonal Antibody #33648

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Product Specifications

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	82
Source/Isotype	Mouse IgG1 kappa

Application Key:

WB-Western Blotting
IP-Immunoprecipitation

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Plakophilin 1 (19F10) Mouse Monoclonal Antibody recognizes endogenous levels of total plakophilin 1 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human plakophilin 1 protein.

Background

Plakophilin 1 (PKP1), a member of the armadillo repeat protein family, is a desmosomal plaque protein predominantly expressed in the suprabasal layers of stratified and complex epithelia, where it plays a central role in maintaining intercellular adhesion and epithelial integrity in tissues frequently exposed to mechanical stress (1). Plakophilin 1 plays a role in modulating several key signaling pathways, including MAPK, Wnt, Akt, and cytoskeletal (2). Plakophilin 1 has a dual role in cancer, functioning as either a tumor suppressor or an oncogene depending on its subcellular localization. When membrane-bound, it stabilizes cell-cell adhesion and may suppress cellular invasion; however, when localized to the cytoplasm or nucleus, it drives oncogenesis by promoting MYC translation and modulating metabolic and DNA damage responses (2,3). PKP1 mutation disrupts desmosomal integrity, causing ectodermal dysplasia-skin fragility syndrome, a rare autosomal recessive disorder characterized by epidermal fragility and abnormal hair and nail growth (4). Due to its diverse biological functions and disease-associated expression patterns, plakophilin 1 is under investigation as both a potential biomarker and therapeutic target.

Alternate Names

B6P; Band 6 protein; EDSFS; MGC138829; PKP1; plakophilin 1; plakophilin 1 (ectodermal dysplasia/skin fragility syndrome); Plakophilin-1

Database Links

UniProt ID: Q13835

Entrez-Gene Id: 5317

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