GST (26H1) Mouse mAb (Sepharose Bead Conjugate) #3513
|3513S||400 µl (40 immunoprecipitations)||---||In Stock||---|
|3513||carrier free and custom formulation / quantity||email request|
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|IP||1:20||Transfected Only||Mouse IgG2a|
Applications Key: IP=Immunoprecipitation
Specificity / Sensitivity
GST (26H1) Mouse mAb (Sepharose Bead Conjugate) detects transfected GST fusion proteins.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a GST fusion protein.
Immunoprecipitation of extracts from COS cells transfected with a construct overexpressing TrCp1-GST using GST (26H1) Mouse mAb (Sepharose Bead Conjugate) (lane 2). Mouse IgG Isotype Control (Sepharose Bead Conjugate) #3420 (lane 1) was used as a negative control. The western blot was probed using GST (91G1) Rabbit mAb #2625.
This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. GST (26H1) Mouse mAb (Sepharose Bead Conjugate) is useful for immunoprecipitation assays.
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.
Glutathione S-transferase (GST) is a widely used fusion partner, since it provides both an easily detectable Tag and a simple purification process with little effect on the biological function of the protein of interest. Numerous vectors containing GST-Tag have been developed for both prokaryotic and eukaryotic systems over the past decade (1-3).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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