Revision 1

#35981

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IP, IHC-P, FC-FP

Reactivity:
H

Sensitivity:
Endogenous

MW (kDa):
26

Source/Isotype:
Rabbit IgG

UniProt ID:
#Q5HYN5

Entrez-Gene Id:
541466

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:200
Immunohistochemistry (Paraffin) 1:4000
Flow Cytometry (Fixed/Permeabilized) 1:2400 - 1:9600

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity/Sensitivity

CT45 (F7S2I) Rabbit Monoclonal Antibody recognizes endogenous levels of total CT45 protein. This antibody is predicted to cross-react with all CT45 family members.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val15 of human CT45-1 protein.

Background

Cancer/testis antigens (CTAs) are a family of more than 100 proteins whose normal expression is largely restricted to immune-privileged germ cells of the testis, ovary, and trophoblast cells of the placenta. Although most normal somatic tissues are void of CTA expression, due to epigenetic silencing of gene expression, their expression is upregulated in a wide variety of human solid and liquid tumors (1,2). As such, CTAs have garnered much attention as attractive targets for a variety of immunotherapy-based approaches to selectively attack tumors (3).

The CT45 family is a tandem cluster of highly similar genes that share many features with other classic CT genes, including Xq localization, multigene family, and identical or near-identical gene copies, indicating recent gene duplications (4). The nuclear protein antigen CT45 is often highly expressed in lung cancer and ovarian cancer, and the frequency and characteristics of CT45 expression are similar to those of other CT cancer vaccine targets currently in clinical trials, including NY-ESO-1 and MAGE-A (5). Overexpression of CT45A1 in breast cancer cells was found to upregulate various oncogenic and metastatic genes, promote epithelial-mesenchymal transition, and increase cell stemness, tumorigenesis, and metastasis (6). Additionally, research studies have shown that CT45A1 silencing suppresses cell proliferation and metastasis in breast and lung cancer cells by downregulating the ERK/CREB signaling pathway (6,7). Therefore, CT45A1 and other CT45 family members are excellent targets for anticancer drug discovery and targeted tumor therapy.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting IP: Immunoprecipitation IHC-P: Immunohistochemistry (Paraffin) FC-FP: Flow Cytometry (Fixed/Permeabilized)

Cross-Reactivity Key

H: Human

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of extracts from various cell lines using CT45 (F7S2I) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Negative expression of CT45 protein in DU 145, MCF7, and SK-MEL-28 cells is consistent with the predicted expression pattern.
Western Blotting Image 1: CT45 (F7S2I) Rabbit Monoclonal Antibody
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length CT45-1 (CT45-1-Myc/DDK; +), using CT45 (F7S2I) Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Western Blotting Image 2: CT45 (F7S2I) Rabbit Monoclonal Antibody
Immunoprecipitation of CT45 protein from A-375 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is CT45 (F7S2I) Rabbit mAb. Western blot analysis was performed using CT45 (E2S3Q) Rabbit mAb #41390. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used as a secondary antibody.
Immunoprecipitation Image 1: CT45 (F7S2I) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human esophageal carcinoma using CT45 (F7S2I) Rabbit mAb.
Immunohistochemistry Image 1: CT45 (F7S2I) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded human serous papillary carcinoma of the ovary using CT45 (F7S2I) Rabbit mAb.
Immunohistochemistry Image 2: CT45 (F7S2I) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded normal human testis using CT45 (F7S2I) Rabbit mAb.
Immunohistochemistry Image 3: CT45 (F7S2I) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human esophageal carcinoma using CT45 (F7S2I) Rabbit mAb (left) or a CT45 Rabbit mAb (right). These two antibodies detect unique, non-overlapping epitopes on CT45. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining.
Immunohistochemistry Image 4: CT45 (F7S2I) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded human esophageal carcinoma using CT45 (F7S2I) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Immunohistochemistry Image 5: CT45 (F7S2I) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded HCC1187 cell pellet (left, positive) or DU 145 cell pellet (right, negative) using CT45 (F7S2I) Rabbit mAb.
Immunohistochemistry Image 6: CT45 (F7S2I) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Flow cytometric analysis of fixed and permeabilized MCF7 cells (blue, negative) and A-375 cells (green, positive) using CT45 (F7S2I) Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Flow Cytometry Image 1: CT45 (F7S2I) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.