Product Pathways - Stem Cell and Lineage Markers
Sox2 (C70B1) Rabbit mAb (IHC Preferred) #3728
|3728S||100 µl (250 sections)||---||In Stock||---|
|3728||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin)
Specificity / Sensitivity
Sox2 (C70B1) Rabbit mAb (IHC Preferred) detects endogenous levels of Sox2 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino acid sequences at the carboxy-terminal region of mouse Sox2.
Western blot analysis of extracts from mouse embryonic stem cells (mESCs) and F9 cells using Sox2 (C70B1) Rabbit mAb (IHC Preferred).
Immunohistochemical analysis of paraffin-embedded F9 cells (positive, left) and C2C12 cells (negative, right) using Sox2 (C70B1) Rabbit mAb (IHC Preferred).
Immunohistochemical analysis of paraffin-embedded 14.5 day mouse embryo using Sox2 (C70B1) Rabbit mAb (IHC Preferred). Note staining of ventricular/subventricular zone.
Embryonic stem cells are derived from the inner cell mass of the blastocyst and are unique in their pluripotent capacity and potential for self-renewal. Sox2 is one of a set of transcription factors that are crucial for the maintenance of pluripotency (1). Sox2, Oct-4, and Nanog cooperate in this network (1-3), and siRNA knockdown of either Sox2 or Oct-4 results in loss of pluripotency (4,5). Chromatin immunoprecipitation experiments have shown that Sox2 and Oct-4 bind to thousands of gene regulatory sites, highlighting the importance of these transcription factors in early embryonic development (6,7). It has recently been shown that Sox2 is amplified in lung and esophageal squamous cell tumors (8).
- Nichols, J. et al. (1998) Cell 95, 379-391.
- Avilion, A.A. et al. (2003) Genes Dev. 17, 126-140.
- Rodda, D.J. et al. (2005) J. Biol. Chem. 280, 24731-24737.
- Matin, M.M. et al. (2004) Stem Cells 22, 659-668.
- Niwa, H. et al. (2000) Nat. Genet. 24, 372-376.
- Boyer, L.A. et al. (2005) Cell 122, 947-956.
- Loh, Y.H. et al. (2006) Nat. Genet. 38, 431-440.
- Bass, A.J. et al. (2009) Nat Genet 41, 1238-42.
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
This antibody is developed, validated, and produced by CST using in part technology under license (granting certain rights including those under U.S. Patent No. 5,675,063) from Epitomics, Inc.