Product Pathways - Cytoskeletal Signaling
Talin-1 (C45F1) Rabbit mAb #4021
|4021S||100 µl (10 western blots)||---||In Stock||---|
|4021||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Hamster, Monkey||Endogenous||270||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
Talin-1 (C45F1) Rabbit mAb recognizes endogenous levels of total Talin-1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Talin-1.
Focal adhesions connect the cytoskeleton with the extracellular matrix (ECM), a complex structure of secreted macromolecules that surrounds mammalian organs and tissues. Integrins clustered on the extracellular side of focal adhesions signal from the ECM to intracellular protein complexes, which in turn signal to the actin cytoskeleton to regulate the tension needed for cell motility. Internal signals also converge on focal adhesions to regulate integrin affinity and avidity. Signaling through focal adhesions regulates cell adhesion, migration, proliferation, apoptosis, and gene expression, and impacts cellular processes such as development, wound healing, immune response, invasion, metastasis, and angiogenesis (reviewed in 1-3). Talin is a large, multidomain focal adhesion protein that interacts with the intracellular domains of integrins and other focal adhesion proteins. Talin is involved in the formation of focal adhesions and in linking focal adhesions to the actin cytoskeleton (4). The interaction between talin and integrins increases the affinity between integrin and both insoluble and soluble ECM proteins (5,6).
- Burridge, K. et al. (1988) Annu Rev Cell Biol 4, 487-525.
- Calderwood, D.A. et al. (2000) J Biol Chem 275, 22607-10.
- ffrench-Constant, C. and Colognato, H. (2004) Trends Cell Biol 14, 678-86.
- Nayal, A. et al. (2004) Curr Opin Cell Biol 16, 94-8.
- Tanentzapf, G. and Brown, N.H. (2006) Nat Cell Biol 8, 601-6.
- Tadokoro, S. et al. (2003) Science 302, 103-6.
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
This antibody is developed, validated, and produced by CST using in part technology under license (granting certain rights including those under U.S. Patent No. 5,675,063) from Epitomics, Inc.